Local action of inhibin dimmer and α-monomer

抑制素二聚体抑制原代培养的垂体前叶细胞促性腺激素的分泌，抑制素α单体对促性腺激素的分泌无影响。但到目前为止，抑制素的作用在不同的组织中是不同的。为了建立一个合适的系统来研究抑制素的作用，我们用激活素对几个细胞进行了研究，因为抑制素的可用性非常有限，而且抑制素和激活素使用共同的受体。(1)垂体细胞：观察激活素对大鼠垂体细胞系分泌催乳素的调节作用，细胞表面有I型和II型激活素受体复合体。激活素改变催乳素分泌。进一步研究抑制素/激活素信号在PRL产生中的作用对我们的研究是有用的。(2)卵巢细胞：DES诱导的幼鼠卵巢颗粒细胞和幼鼠卵母细胞-颗粒-膜细胞复合体对低浓度激活素有反应。因此，这些细胞似乎适合于研究抑制素二聚体和α-单体的作用。(3)胎盘细胞：对人足月胎盘细胞进行原代培养。只有高浓度的激活素才能改变hCG的分泌，因此这些细胞似乎不适合用来研究抑制素的局部作用。

Inhibin dimmer inhibits FSH secretion from anterior pituitary cells in primary culture and inhibin α-monomer has no effect on FSH secretion. But as reported so far, the action of inhibin is different from tissue to tissue. In order to establish a suitable system to investigate the action of inhibin, we investigated several cells using activin because the availability of inhibin is very limited and inhibin and activin use common receptors. (1) Pituitary cells : The regulation of PRL secretion by activin from a rat pituitary cell line with type-I and type-II activin receptor complex on the cell surface was investigated. PRL secretion was changed by activin. Further study regarding the involvement of the inhibin/activin signal in PRL production is useful for our study. (2) Ovarian cells : Granulosa cells from DES-primed immature rats and oocyte-granulosa-theca cell complex from immature mice reacted to activin in low concentrations. Therefore these cells seem to be suitable in order to study the action of inhibin dimmer and α-monomer. (3) Placental cells : A primary culture of human placental cells at term was investigated. Only high concentrations of activin changed the secretion of hCG so these cells do not seem suitable to use to investigate the local action of inhibin.