Induction of anti- Porphyromanas gingivalis antibody by tonsillar application

扁桃体贴敷是一种新的免疫方法，可诱导唾液和血浆中抗原产生特异性抗体。我们将福尔马林灭活的牙龈卟啉单胞菌（Porphyromonas gingivaris，P.gingivalis）细胞应用于扁桃体，获得了以下结果.结果：1.应用灭活的牙龈卟啉单胞菌诱导唾液中抗牙龈卟啉单胞菌伊加和血浆中抗牙龈卟啉单胞菌IgG的产生.扁桃体贴敷后，腮腺周围组织中抗牙龈卟啉单胞菌伊加产生细胞增多.诱导的唾液伊加对牙龈卟啉单胞菌的应用菌株和其他几种不同的菌株都有反应。对中间普雷沃氏菌、具核梭杆菌、血链球菌的反应水平很低.诱导的唾液伊加可抑制牙龈卟啉单胞菌与牙龈上皮细胞克隆细胞的粘附，提示牙龈卟啉单胞菌灭活细胞扁桃体给药可诱导唾液中产生抗原特异性抗体。由于诱导的抗体抑制牙龈卟啉单胞菌对牙龈上皮细胞的粘附，这表明牙周病细菌细胞的扁桃体应用是可能的，以预防牙周病。

Tonsillar application is a new immunization method for inducing a specific antibody against antigen in saliva and blood plasma. We tonsillarly applied formalin-killed cells of Porphyromonas gingivaris (P.gingivalis), a periodontopathic bacteria, and obtained following results.1. Tosillar application of killed P. gingivalis induced anti-P. gingivalis IgA in saliva and anti-P. gingivalis IgG in blood plasma.2. A number of anti-P. gingivalis IgA produsing cells increased in the tissue surrounding parotid gland by tonsillar application.3. Induced salivary IgA reacted against P. gingivalis both the applied strain and other several kind of the different strain. The reactions to Prevotella intermedia, Fusobacterium nucleatum, and Streptococcus sanguis were very low level.4. Induced salivary IgA inhibited the adherence of P. gingivalis to the cloned cells of gingival epithelial cell in vitro.These results shows that tonsillar application by killed cell of P. gingivalis induced antigen specific antibody in saliva. Since the induced antibody inhibited the adherence of P. gingivalis to gingival epithelial cells, it is suggested that tonsillar application of the periodontpathic bacterial cells is possible to prevent periodontal disease.