Alteration of salivary IgA level due to surgical stress

手术应激导致唾液 IgA 水平发生变化

• 批准号: 14571921
• 负责人: NAKAGAWA Yoichi
• 金额: $ 1.79万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571921/
• 关键词: IgA; Saliva; Surgery; Stress; Mouse; Salivary gland; 外科侵襲; 交感神経

It was shown that surgical insult exerts various changes on an immunity system, which include reduction of the number of peripheral lymphocytes, reduction of natural killer activity, change of an immunoglobulin. The purpose of the study is to examine the influence on the membrane immunity due to surgical insult. Quantitative change of secretion of salivary IgA, and change of the amount of IgA production in the salivary gland were examined.(Materials and Method) (1) Operation was carried out under general anesthesia using the ICR male mice. A combination of a skin incision and development of a flap was employed as a surgical insult. Collection of saliva was performed with administration of pilocarpine (muscarinic receptor agonist), and saliva was collected with micro pipette from the mouth. Untreated mice were used for saliva collection under pentobarbital anesthesia as a control. (2) IgA concentration of saliva sample was determined by ELISA. (3) IgA mRNA was determined by competitive PCR.(Results) The IgA concentration in the saliva rose immediately after surgery, ad returned to the normal value after the next day. When this value was calculated as IgA concentration per saliva protein, it showed tendency to be high on the next day, but not high immediately after surgery. This result suggests that IgA was working for body defense against the surgical insult.The amount of IgA mRNA in the submandibular gland was increased immediately after surgery.(Conclusion) It was suggested that increase of production and secretion of IgA was induced by the condition that the sympathetic nerve was predominant due t surgical insult.

研究表明，手术损伤会对免疫系统产生各种变化，包括外周淋巴细胞数量减少、自然杀伤活性降低、免疫球蛋白变化。该研究的目的是检查手术损伤对膜免疫的影响。检查唾液IgA分泌量的变化以及唾液腺中IgA产生量的变化。 (材料和方法) (1)使用ICR雄性小鼠在全身麻醉下进行手术。采用皮肤切口和皮瓣发育的组合作为手术损伤。通过给予毛果芸香碱(毒蕈碱受体激动剂)来收集唾液，并用微量吸管从口腔中收集唾液。未处理的小鼠在戊巴比妥麻醉下用于唾液收集作为对照。 (2)采用ELISA法测定唾液样品的IgA浓度。 (3)竞争性PCR法测定IgA mRNA。(结果)术后唾液中IgA浓度立即上升,次日恢复正常值。当将该值计算为每个唾液蛋白的 IgA 浓度时，显示出第二天较高的趋势，但手术后立即并不高。这一结果表明，IgA 正在发挥身体防御手术损伤的作用。手术后下颌下腺中 IgA mRNA 的量立即增加。（结论）提示 IgA 的产生和分泌增加是由于手术损伤导致交感神经占主导地位而引起的。