Experimental studies on the injection of adriamycia into a third division of trigeminal nerve

本研究根据三叉神经痛的治疗方法，在三叉神经末梢注射ADM，经逆行轴浆运输将ADM转运至神经节，进行组织学和电生理研究。组织学研究(1)荧光显微镜观察：选用Wistar系雌性大鼠。全麻（腹腔注射戊巴比妥钠）下肺泡下神经暴露于颏孔处。用微注射器将5%、1%、0.5%、0.1%浓度的ADM 10μ1注射到每只大鼠的神经中。分别于12小时、18小时和24小时后灌注大鼠。三叉神经节被切除并熄灭。在冷冻切片上切20 μm厚的切片。在荧光显微镜下观察ADM的自身荧光。结果，5% ADM注射12 h后，观察到ADM的自荧光。注射1% ADM后18h，观察到较弱的自发荧光。这些显示ADM被运输到三叉神经节。(2)组织病理学结果：注射ADM后，分别于24小时、7天、21天切除三叉神经节并包埋石蜡。6 μm厚的切片采用苏木精和伊红染色，Kluver- Barrera染色。注射5% ADM后7 d，神经节细胞出现空泡，染色质溶解，细胞核移位。21 d后外核溶解，呈篮状。神经节细胞呈核固缩或核分裂。电生理研究：在颏孔处暴露下牙槽神经，用电刺激系统刺激下牙槽神经，记录诱发电位。其余大鼠神经注射5% ADM。24小时、7天、21天分别记录诱发电位。注射后24 h，诱发电位明显低于未注射大鼠。第7天和第21天不记录诱发电位的形态。因此，这些结果表明，该方法可以选择性地破坏三叉神经节细胞。少

For the study according to the treatment of trigeminal neuralgia, we carried out the histological and electrophysiplogical study with injecting ADM to the trigeminal nerve terminal and transporting ADM to the ganglion by retrograde axoplasmic transport.1. Histological Study (1)Fluorescence microscopic Observation : We used female Wistar strain rats. Under general anesthesia (intraperitoneal injection of sodium pentobarbital), the inferior alveolar nerve was exposed at the mental foramen.10μ1 of a 5 %, 1 %, 0.5 %, 0.1 % concentration of ADM was injected into the nerve of each rat with a microsyringe. After 12 hours, 18 hours and 24 hours, the rat was perfused.The trigeminal ganglion was removed and quenched. 20 μm-thick sections were cut on a cryomicrotome.The specimens were examined with a fluorescence microscope to observe autofluorescence of ADM.. In result, 12 hours after 5 % ADM iniection, auto fluorescence of ADM was observed. And 18 hours after 1 % ADM mjection, weak auto fluores … More cence was observed. These showed ADM was transported to the trigeminal ganglion. (2)Histopathological findings : After injection of ADM, the trigeminal ganglion was removed (after 24 hours,7 days, and 21 days) and parafin-embedded.6 μm-thick sections were stained by the hematoxylin ana eosin, Kluver- Barrera methods.7 days after injection of 5 %ADM, ganglion cells showed vacuoles, chromatolysis, and displacement of nucleus.21 days after, perikaryon was dissolved and showed basket-like form. Gangion cells showed karyopyKnosis or karyoclasis.2. Electrophysiological study : The inferior alveolar nerve was exposed at the mental foramen : The nerve was stimulated by electric stimulation system and evoked potentials were recorded. Other rats were injected 5 % ADM to the nerve. After 24 hours, 7 days, and 21 days, the evoked potentials were recorded.24 hours after injection, evoked potentials were much lowwer than the potentials of no-injection rat. After 7days and 21days,the were form of evoked potentials were not recorded. Therefore, these results suggest that the method can destroy the trigeminal ganglion cells selectively. Less