STRUCTURE AND FUNCTION OF ANIMAL ION TRANSPORTER THAT EXPRESS IN BACTERIA

小鼠TRP（瞬时受体电位）同源物作为阳离子通道发挥作用，通过Ca^2+和Na^+。当TRP同源基因转化到缺乏所有Na^+/H^+反向转运蛋白的大肠杆菌突变株TO 114中时，该菌株变得对Na^+敏感，这表明它在大肠杆菌膜中起作用。我们检测到His标记的TRP同源物在大肠杆菌的膜部分中表达。TRP可能与辣椒素受体VR 1具有共同的结构，但这些结构尚不清楚。TRP/VR 1被认为与具有6个跨膜区的Shaker型K^+通道具有同源性，但还没有实验报道。我们利用细菌系统进行了TRP结构的实验，并得到了一些结果。我们正在用哺乳动物培养细胞做进一步的实验，以证实从细菌系统中得到的TRP结构。

TRP (transient receptor rotential) homologue of mouse function as cation channel that passes Ca^<2+> and Na^+. When TRP homologue gene was transformed in Escherichia coli mutant strain TO114 which lacks all Na^+/H^+ antiporters, this strain became sensitive to Na^+ suggesting that it functions in E.coli membrane. We detected His tagged TRP homologue expressed in membrane fraction of E.coli. TRP probably has common structure with VR1, capsaicin receptor, but those structures are not known. TRP/VR1 were suggested to have homology with Shaker type K^+ channels with 6 transmembrane regions, though there are no experimental reports. We started experiment for TRP structure using bacterial system and got some results. We are doing further experiment using mammalian cultured cells to confirm the TRP structure that got from bacterial system.