Highly sensitive analysis of the constituents in whole blood diluted with chemiluminescence-FIA using a bioreactor

采用化学发光检测器（光子计数型）和固定化酶柱反应器组成的FIA（流动注射分析）系统，对稀释后的全血中的葡萄糖、尿酸等成分进行了高灵敏度分析，使蚊子抽取的血容量水平能够测定血液中的许多成分。葡萄糖测定-全血用生理盐水或蒸馏水稀释，血浆用蒸馏水稀释至5000 ~ 400000倍。使用fia -化学发光系统测定稀释样品中的葡萄糖，该系统涉及吡喃糖氧化酶柱反应器（2x20 mm）。样品进样量为10 μ l，分配器（Accu-drive, made in Jamany）的使用和蒸馏水在玻璃容器中保存有助于显著提高葡萄糖测定的重复性。结果表明，即使将样品稀释到10万倍，其重现性仍令人满意。用全血预先用蒸馏水或生理盐水稀释至10万倍，用accu驱动分配器测得葡萄糖浓度，并与葡萄糖氧化酶-过氧化氢电极法测得葡萄糖浓度进行比较。蒸馏水计算得到的线性回归和相关系数分别为y=0.916x-4.05和r=0.986，生理盐水计算得到的线性回归和相关系数分别为y=0.910x-3.00和r=0.988，说明两种方法的结果具有良好的相关性。对于用蒸馏水稀释至10万倍的血浆，计算出的线性回归和相关系数为y=0.979x-3.1, r=0.987，表明两种方法的结果在较宽的葡萄糖浓度范围内非常吻合。

Highly sensitive analysis of the constituents (glucose, uric acid) in whole blood diluted was tried using FIA (flow injection analysis) system involving a chemiluminescence detector (photon counting type) and an immobilized enzyme column reactor, so that a lot of constituents in blood were able to be determined with blood volume level which a mosquito drew.1. Glucose determination-Beforehand, whole blood was diluted with physiological saline solution or distilled water, and plasma with distilled water to 5000〜400000-fold. Glucose in the diluted samples was determinated using the FIA-chemiluminescence system involving a pyranose oxidase column reactor (2x20 mm). The sample volume injected was 10 μ l. The use of a dispenser (Accu-drive, made in Jamany) and the preservation of distilled water in a glass container contributed to improve the reproducibility of glucose determination sharply. As the resuly, the reproducibility was satisfactory even the sample diluted to a hundred thousand-fold. The results of glucose concentration obtained by this method using whole blood diluted beforehand with distilled water or physiological saline solution to a hundred thousand-fold by a Accu-drive dispenser were compared with those obtained with the glucose oxidase-hydrogen peroxide electrode method. The calculated linear regression and correlation coefficient were y=0.916x-4.05 and r=0.986 for distilled water, and y=0.910x-3.00 and r=0.988 for physiological saline solution, indicating excellent correlation between the results of the two methods. In the case of plasma diluted with distilled water to a hundred thousand-fold, the calculated linear regression and correlation coefficient were y=0.979x-3.1 and r=0.987, indicating excellent agreement between the results of the two methods over a wide range of glucose concentrations.