"Development of chiral-selective amine acid sensors"

以l -谷氨酸为模板的过氧化聚吡咯膜已被用于l -谷氨酸和d -谷氨酸的对映体分离。利用荧光光谱法结合电化学石英晶体微天平技术，研究了控制过氧化聚吡咯薄膜性能的各种重要制备因素。测量到的荧光强度与吸收到膜中的谷氨酸浓度有关。结果发现，l -谷氨酸在薄膜中的插入量是d -谷氨酸的30倍左右。通过改变氨基酸溶液的应用电位和pH等关键参数，使传感器的响应达到最佳。以l -谷氨酸为模板的传感器对其他几种l -和d -氨基酸也表现出优异的选择性。与先前报道的印迹聚合物相比，过度氧化的聚吡咯膜具有更高的对映选择性，这可归因于潜在诱导的目标分子的摄取/释放。

Overoxidized polypyrrole films templated with L-glutamate have been utilized for enantiomeric separation of L-and D-glutamic acid. Various important fabrication factors controlling the performance of the overoxidized polypyrrole films have been investigated using fluorescence spectrometry in conjugation with the electrochemical quartz crystal microbalance technique. The measured fluorescence intensity was related to the concentration of glutamate taken up into the films. It was found that L-glutamate was inserted about 30 times as much into the film as D-glutamate. Several key parameters such as applied potential, and pH of amino acid solution were varied so as to achieve the optimum sensor response. The sensor templated with L-glutamic acid also exhibited excellent selectivity over several other L-and D-amino acids. Higher enantioselectivity of overoxidized polypyrrole film than that for previously reported imprinted polymers can be attributed to the potential-induced uptake/release of targeted molecules.