Study of fish cultivation using carotenoids supplemented diets without medication

用添加类胡萝卜素(β-胡萝卜素或虾青素)的饲料和未添加的对照饲料，分别在孵化后20天和28天，不加药的情况下，饲喂红鱼和虎河豚幼体。结果表明，添加类胡萝卜素的红鱼和河豚的成活率均高于各对照组。在本实验中，添加类胡萝卜素和不添加类胡萝卜素的对照组在两个物种中表现出类似的生长。添加胡萝卜素或虾青素的β-胡萝卜素或虾青素处理组的脾细胞在20 mgg/ml巴豆草有丝分裂原或100 mgCon A处理后，其增殖反应均高于对照组。在虎河豚中，用20μg/ml的商陆有丝分裂原或100μg/ml的ConA处理来自β-胡萝卜素组的脾细胞。淋巴细胞增殖程度明显高于对照组。用添加β-胡萝卜素的日粮和不添加任何药物的对照日粮饲养1岁黄尾大鼠2~3个月。结果表明，添加β-胡萝卜素的对照组和添加β-胡萝卜素的对照组生长发育相似，血清Ht、Hb、GOT、GPT水平相近。在饲料中添加20μg/ml的商陆丝裂原或100μg/ml的刀豆球蛋白A(ConA)处理组，脾细胞的增殖率明显高于对照组。结果表明，在饲料中添加类胡萝卜素(β-胡萝卜素或虾青素)，可在不加药的情况下生产出抗感染性强的健康鱼类。

Red sea bream and tiger puffer larvae were fed with carotenoids(β-carotene or astaxanthin) supplemented diets or unsupplemented control diet for 20 and 28 days after hatchout, respectively without medication. Results show that survival rates of carotenoids supplemented groups of both red sea bream and tiger puffer were higher than that of each control group. The carotenoids supplemented and unsupplemented control groups exhibited a similar growth in both species during this experiment. The proliferation of splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg/ml of concanavalin A (Con A) from β-carotene supplemented or astaxanthin supplemented group were higher than that of a control group in red sea bream. In tiger puffer, splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg /ml of Con A from the β-carotene-supplemented group. The lymphocytes proliferated to a higher degree compared to the control. Yellowtail (1-year-old) were fed with β-carotene-supplemented diets or unsupplemented control diet for 2-3 months without medication. Results show that the p-carotene-supplemented and unsupplemented control groups exhibited a similar growth and a similar serum values in Ht, Hb, GOT, GPT during this experiment. The proliferation of splenocytes treated with 20 μg/ml of poke weed mitogen or 100 μg/ml of concanavalin A (Con A) from p-carotene supplemented group was higher than that of a control group.Results suggested that the supplementation of carotenoids (β-carotene or astaxanthin) to diets, might be of benefit in production of healthy and high resistive fishes against infectious disease without medication.