The development studies on the peripheral nervous systems and muscles used LacZ gene recombinant adenovirus

神经与肌肉之间有着密切的一对一关系。为了生存，相互依存是必要的。至今仍未解决如此密切的关系。此外，标签在神经研究中是必要的。本研究以LacZ基因重组腺病毒为新细胞标记材料，阐明了周围神经与肌肉的胚胎学关系。小鸡胚胎被用作材料，首先，研究人员检查了神经如何在病毒中被标记。然后观察外周神经在肢芽内的形成过程，并寻找延伸神经纤维的位置结构。此外，我们还观察了受体型扭转激酶及其配体的表达模式，以阐明与神经分支有关的肢芽分子环境。此外，在肢芽肌肉祖细胞的迁移和个体肌肉块的形成过程中，神经与肌肉之间存在怎样的关系？LacZ基因重组腺病毒是神经系统发育研究较为方便的标记。感觉神经和运动神经在相同的环境下伸展它们的纤维。结果表明，受体型扭转激酶及其配体在肢芽中呈现背-腹互补表达模式，分别诱导周围神经纤维束的背支和腹支。在这个时候，血管似乎成为了一个屏障，提高了方向决定的准确性。很明显，神经和肌肉的发育并没有直接的关系，它们各自独立地推进自己的发育过程。

Nerve and muscle have the close relationship between the one-to-one. In order to survive, the mutuality is required. It has not been solved still such close relationships. In addition, labels are necessary to the neuroresearches. In this study, LacZ gene recombination adenovirus was used as a material of new cell marking and clarified the embryological relation between the peripheral nerves and the muscles.The chick embryos were used as a material, and at first, how the nerve was labeled in the virus, was examined. Then, the formation process of peripheral nerve in limb bud was observed, and the structure of the positions which extended the nerve fibers was searched. Further, the expression patterns of receptor type torsion kinase and its ligand was observed in order to elucidate the molecular environment in the limb buds concerning the branching of nerves. In addition, what kind of relation was observed between the nerves and the muscles during the migration of muscle progenitor cells in the limb bud and the formation of the individual muscle mass.LacZ gene recombination adenovirus is the comparatively convenient mark for the developmental research on the nervous system. The sensory and motor nerves extended their fibers using the same environment. It was suggested that the receptor type torsion kinase and its ligand, which showed the dorsal-ventral complementary expression pattern in the limb bud induced the dorsal and ventral branches of the peripheral nerve fiber bundles, respectively. In this time, it seems that the blood vessel became a barrier and raised the accuracy of the direction decision. It became clear that the development of the nerves and muscles did not have the direct relationship, and they advanced their developmental processes independently.