Study on apoprotein of the convulsion-inducing factor in schistosome egg granulomas

从感染曼氏血吸虫的小鼠肝卵肉芽肿和脾脏中纯化了一种致痫脂蛋白（CILIP），并对其理化性质进行了表征。根据所建立的CILIP纯化方法，还可以从未感染和感染mansoni的小鼠、几种近交菌株和SPF条件下免疫功能低下的小鼠、大鼠、兔和人的各种组织中纯化CILIP样惊厥诱导物质。这些物质表现出与CILIP相同的物理和化学性质。静脉注射这些物质所引起的体征和症状与注射CILIP所显示的相同。此外，体内研究表明，这些物质可能通过与CILIP相同的机制起作用。因此，这些物质被认为是与CILIP相容的脂蛋白。因此，CILIP可能广泛分布于各种动物和人体组织中。兔和小鼠CILIP多克隆抗体不仅能完全抑制CILIP活性，还能抑制从动物和人体组织中分离的惊厥诱导物质的活性。这一证据表明，在这些诱发抽搐的脂蛋白中有一种共同的分子。二维电泳证实了从动物和人体组织中分离的惊厥诱导脂蛋白中存在两种常见分子。所有这些证据表明，两种常见肽可能在CILIP的药理作用中起重要作用。

A convulsion-inducing lipoprotein (CILIP) was purified from hepatic egg granulomas and spleens of mice infected with Schistosoma mansoni, and its physical and chemical properties were characterized. The CILIP-like convulsion-inducing substances could be also purified from various tissues of uninfected and infected mice with S.mansoni, several inbred strains and immunocompromised mice cared under SPF condition, rat, rabbit, and human according to the method developed for CILIP purification. These substances showed the same physical and chemical properties as CILIP.Intravenous injection of these substances caused the same sign and symptoms as have been demonstrated by CILIP injection. Moreover, in vivo study indicates that these substances may act through the same mechanism as CILIP.These substances are, therefore, considered to be compatible lipoproteins with CILIP.It is, therefore, likely that CILIP may be widely distributed in various animal and human tissues.Rabbit and mouse polyclonal antibody to CILIP completely inhibited not only CILIP activity but also the activities of convulsion-inducing substances isolated from various animal and human tissues. This evidence suggests that there are a common molecule among these convulsion-inducing lipoproteins. The existence of two types of common molecules in the convulsion-inducing lipoproteins isolated from various animal and human tissues was confirmed by two dimensional electrophoresis. All of these evidences suggest that two types of common peptides probably play an essential role in the pharmacological action of CILIP.