Basic study on Enhancement Effect on DNA vaccine in Hepatitis type C

我们试图通过使用DNA疫苗在小鼠中诱导对丙型肝炎病毒（HCV）NS 5A蛋白的T细胞应答。我们用单独编码HCV NS 5A区的质粒或用编码IL-2、GM-CSF或CD 86的佐剂质粒免疫小鼠。用IL-4和GM-CSF从骨髓细胞中诱导成熟的树突状细胞。我们免疫小鼠与树突状细胞脉冲与据报道免疫原性CTL表位。这些小鼠都没有表现出更好的T细胞反应。

We tried to induce a T cell response to hepatitic C virus (HCV) NS5A protein by using a DNA vaccine in mice. We immunized mice with a plasmid encoding HCV NS5A region alone or with an adjuvant plasmid encoding IL-2, GM-CSF, or CD86. Neither of them induced a cytotoxic T lymphocyte (CTL) response of a siginificant magnitude.Next we induced mature dendritic cells with IL-4 and GM-CSF from bone marrow cells. We immunized mice with dencritic cells pulsed with reportedly immunogenic CTL epitopes. None of these mice showed a better T cell response yet.