Research of chemokines and platelet-derived microparticles in progression of thrombotic vascuritis

利用流式细胞术和激光共聚焦扫描显微镜观察高剪切诱导血小板和微粒活化对THP-1和内皮细胞粘附分子的影响。我们还测量了一些细胞因子的产生，并研究了PMP刺激后THP-1和ECs的细胞因子mRNA。PMP刺激THP-1细胞增加CD11b、CD32和CD33，但不增加CD29、CD31和CD36。PMP刺激ECs增加CD54和CD63，但不增加CD9、CD29和CD31。PMPs诱导THP-1产生IL-8、IL-1β和tnf - α。PMPs还能诱导ECs产生IL-8、IL-1β和IL-6。生产依赖于时间。RT-PCR检测了PMP刺激后THP-1和ECs中部分细胞因子mrna的表达。关于PMP刺激后的粘附性，我们可以清楚地观察到THP-1细胞中CD11b和ECs中CD54的分布发生了变化。为了更好地了解糖尿病患者和健康对照者的pmp和血小板活化标志物（活化血小板上的膜联蛋白V和CD62P）、趋化因子（IL-8、单核细胞趋化肽1[MCP-1]、正常t细胞表达和分泌的活化调节因子[RANTES]）和选择素（p -选择素和e -选择素）的水平，以便更好地了解它们在糖尿病血管并发症中的潜在作用。糖尿病患者pmp、P-选择素、MCP-1、RANTES和可溶性P-和e -选择素水平显著升高，而IL-8水平相似。这些结果表明，高剪切诱导的微颗粒可能有助于动脉粥样硬化的发展，并参与炎症性疾病的血管损伤。

We we used flow cytometry and confocal laser scanning microscopy to investigate the effects of high-shear-induced platelet and microparticle activation in adhesion molecules of THP-1 and endothelial cells (ECs). We also measured the production of some cytokines and studied cytokine mRNA from THP-1 and ECs after PMP stimulation. PMP stimulation of THP-1 cells increased CD11b, CD32, and CD33 but not CD29, CD31, and CD36. PMP stimulation of ECs increased CD54 and CD63 but not CD9, CD29, and CD31. PMPs induced IL-8, IL-1β, and TNFα production by THP-1. PMPs also induced IL-8, IL-1β, and IL-6 production by ECs. Production was time-dependent. With RT-PCR, some cytokine mRNAs were detected in THP-1 and ECs after PMP stimulation. In relation to adhesiveness after PMP stimulation, we could clearly observe a shift in distribution not only of CD11b in THP-1 cells but also of CD54 in ECs.Levels of PMPs and platelet activation markers(Annexin V and CD62P on activated platelets), chemokines(IL-8, monocyte chemotactic peptide 1[MCP-1], and regulated on activation normally T-cell expressed and secreted [RANTES]), and selectins(P-selectin and E-electin)in diabetic patients and healthy control subjects in order to develop a better understanding of their potential contribution to diabetic vascular complications. Significant increases were found for PMPs, P-selectin, MCP-1, RANTES and soluble P- and E-selectins in diabetic individuals, whereas IL-8 levels were similar. These results suggest that high-shear-induced microparticles may contribute to the development of atherosclerosis and participate in vascular damage in inflammatory disorders.