ANALYSIS OF RAT'S BEHAVIOR BY VIDEO AND BRAIN DOPAMIN METABOLISM DURING HYPERPHAGIA INDUCED BY SCHEDULED FEEDING

饮食失调患者不仅存在异常的进食行为，还存在多动、冲动等一般行为的异常。为了阐明这些行为的生物学基础，采用视频图像运动分析系统，观察了限制定时喂养（sf组）、食物剥夺（dp组）和对照组（c组）大鼠的一般行为，包括进食行为、移动时间、移动长度和饲养次数。采用微透析技术测定大鼠纹状体细胞外DA、DOPAC和HVA浓度。在自由饲养前，当它们移动到新环境的监测笼时，sf组的移动时间和时间都有所增加，但持续时间均小于60 min。自由喂养开始后，大鼠的摄食量依次为SF-组、DP-组和c -组。另一方面，分析的任何其他一般行为没有差异。结果表明，两种模式之间的摄食量存在差异。游离饲养后，sf组DA代谢明显高于其他各组。在其他一般行为未发生改变的情况下，DA代谢差异可能与摄食相关因素有关。DP-组和c -组的DA代谢没有差异，尽管食物摄入量不同。这些结果进一步表明，DA代谢的增加不是由摄食行为本身引起的，而是由应激反应等摄食模式引起的。即使在人类饮食失调中，不仅饮食模式的改变，伴随慢性饮食的应激反应也可能与神经递质异常有关。

In patients with eating disorder, not only abnormal feeding behavior but abnormal general behaviors like hyper activity, impulsive behavior are observed. To clarify the biological basis of these behavior, rats with restricted scheduled feeding (SF-group), food deprivation (DP-group), and control (C-group) were observed their general behaviors including eating behavior, moving time, moving length, and number of rearing using a video image motion analysis system. The extracellular concentrations of DA, DOPAC, and HVA were also measured in rat striatum using microdialysis technique. Before free feeding when they moved to the monitor cage which mean new environment, moving time and length were increased in SF-group but it last less than 60 min. After free feeding began, rats ate more food in the order of SF-, DP- and C-group due to their feeding paradigms. On the other hand, any other general behaviors analyzed did not differ. The results suggested that the meal size were changed between the paradigms. DA metabolism in the SF-group was increased after free feeding compared with other groups. As other general behaviors were not changed, the difference in the DA metabolism might be due to feeding related factors. DA metabolism did not differed in DP- and C-group in spite of the different food intake. These results further suggested that the increased DA metabolism was not due to feeding behavior itself, but was due to feeding paradigms like stress reaction. Even in human eating disorders, not only the change of eating pattern but the stress reaction accompany with the chronic diet might related to their neurotransmitter abnormalities.