Direct Gene Transfer Into Articular Cartilage By Gene Gun

目的：探讨基因枪能否将标记基因导入关节软骨。在全髋关节置换术和全膝关节置换术中分别取股骨头和股骨髁的关节软骨。将软骨组织切片，大小为10×10 mm。将表达β-半乳糖苷酶的细菌标记基因lac-Z包被到金粒子上。用Helios基因枪(100psi、200psi、300psi、400psi)轰击关节软骨。取胎盘切片进行器官培养。B-半乳糖苷酶活性在轰击后24~48小时进行测定，24小时后酶活性略高于48小时，但与对照组相比差异不显著。轰击压力不影响β-半乳糖苷酶活性。组织学检查显示，携带lac Z基因的金粒子不能穿透关节软骨。本研究表明，用Helios基因枪进行的粒子轰击不能使金粒子穿透关节软骨组织，因此不能将标记基因转移到关节软骨中。

We investigated whether marker gene can be transferred into the articular cartilage with a gene gun.Articular cartilage was harvested from the femoral head and femoral condyle at the total hip replacement and total knee replacement. Cartilage tissue was dissected into slices, measuring 10×10 mm. A bacterial marker gene, lac Z, which expresses the enzyme β-galactasidase, was coated on gold particle. The articular cartilage was bombared with a Helios Gene Gun (100 psi, 200 psi, 300 psi, 400 psi). The carilage slice was cultured as an organ culture. b-galactasidase activity was evaluated 24 to 48 hours after bombardment.b-galactasidase activity after 24 hours was slightly higher than that after 48 hours, however, those activities were not significantly higher than control. Pressure at the bombardment did not influence the β-galactasidase activity. Histological examination revealed that the gold particles with lac Z gene did not penetrate the articular cartilage.The present study demonstated that the particle bombardment using a Helios Gene Gun cannot transfer the marker gene into the articular cartilage because it cannot make the gold particle penetrate the articular cartilage tissue.