Effect of neuropeptides on postmenopausal bone metabolism

尽管人们对骨质疏松症的兴趣日益增加，并将一些有效的药物引入临床领域，但其病理生理机制尚未阐明。已知许多因素导致骨质疏松症，如雌激素缺乏，许多其他因素也有助于对雌激素的反应。本研究以神经肽受体为研究对象，以小鼠为模型，分析了神经肽受体随激素环境，特别是雌激素环境的变化，采用RT-PCR方法检测了Sham、OVX和E2替代ddY小鼠骨组织中12种神经肽受体mRNA的表达。检测骨组织中VIP-2、CGRP-RCP和ALP受体的表达。OVX组VIP-2的相对表达量是Sham组的1.75倍。有显著差异。E2替代组与Sham组之间无差异。OVX组CGRP-RCP相对表达量为E2替代组的0.55倍。骨组织中的神经肽受体随雌激素水平的变化而变化，提示神经肽在绝经后骨代谢中可能起重要作用。

In spite of increasing interest in osteoporosis and several effective drugs introduced to clinical fields, its pathophysiology is not clarified yet. Many factors are known to contribute to osteoporosis, such as estrogen deficiency, and many other factors also contribute to the response to estrogen. In this study, we paid attention to neuropeptide receptors and analyzed those changes with hormonal environment, especially estrogen with mouse models.Expressions of 12 neuropeptide receptors m-RNA in bone were analyzed on Sham, OVX and E2 replaced ddY mouse with RT-PCR method. The expressions of VIP-2, CGRP-RCP, and ALP receptors were detected on bone. The relative expression of VIP-2 in OVX group was about 1.75 folds higher than that in Sham group. There was a significantly difference. No difference was found between E2 replaced and Sham groups. The relative expression of CGRP-RCP in OVX group was lower (0.55 folds) than that in E2 replaced group. On the other hands, the expression of ALP did not differ among three groups.Some neuropeptide receptors on bone changed with respect to estrogen status and these results suggested that neuropeptides may play an important role in postmenopausal bone metabolism.