Changes in the expression of adenylyl cyclase and G-protein mRNA and the regulation system in myometrium

1腺苷酸环化酶（AC）和G蛋白mRNA在大鼠妊娠期和分娩后子宫肌层的表达● AC（AC 2、AC 4、AC 6、AC 7和AC 9）的表达水平在妊娠期升高，在足月和分娩后降低。各组AC的表达水平均在妊娠第17天达到最高。● Gs蛋白的表达水平仅在妊娠第17天增加，在其他日期没有变化。2妊娠期间和分娩后大鼠子宫肌层中AC的活性●与上述发现一致，基础AC活性和Forcenin刺激的AC活性在妊娠过程中增加，在分娩后和分娩后减少。大鼠去势和激素给药模型中的蛋白质mRNAs ●雌激素（E）、E和孕酮（P）增加AC2、AC4和AC9 mRNAs的表达，AC 6和AC 7 mRNA的表达没有被E或P改变。● Gs mRNA的表达被P增加。4 AC mRNA在仅一侧子宫妊娠的大鼠模型中的表达● AC 2 mRNA在妊娠侧中的表达增加● AC4、AC6、AC7和AC9 mRNA在妊娠侧和非妊娠侧的表达无明显变化。AC和Gs mRNA的表达受性别差异的影响. AC mRNA的表达可能受除雌激素和孕酮以外的多种因素的影响. AC2mRNA的表达主要受局部激素分泌或牵张刺激的诱导。

1 Expression of adenylyl cyclase (AC) and G-protein mRNAs in rat myometrium during the course of pregnancy and after delivery● The level of expression of ACs (AC2, AC4, AC6, AC7 and AC9) increased during the course of pregnancy and diminished near term and after delivery. The highest level of expression in each type of AC was consistently seen on day 17 of pregnancy.● The level of expression of Gs protein increased only on day 17 of pregnancy, and it did not change on the other dates.2 AC activities in rat myometrium during the course of pregnancy and after delivery● In agreement with above findings, both basal and forscolin-stimulated AC activities increased during the course of pregnancy and diminished near term and after delivery.3 Changes in the expression of AC and G-protein mRNAs in rat castrated and hormone administrated model● The expression of AC2, AC4 and AC9 mRNAs increased by estrogen (E), E and progesterone (P) and P, respectively.● The expression of AC6 and AC7 mRNAs did not change by E or P.● The expression of Gs mRNAs increased by P.4 Expression of AC mRNAs in rat model that is pregnant only in the one side of the uterus● The expression of AC2 mRNAs increased in the pregnant side coropared to that in the non-pregnant side.● The expression of AC4, AC6, AC7 and AC9 mRNAs did not change in the both pregnant and non-pregnant sides.These results suggest that1. The expression of ACs and Gs mRNAs is influenced by the different sex hormones.2. There is a possibility that the expression of AC mRNAs is influenced by several factors except to estrogen and progesterone.3. The expression of AC2 mRNA seeros to induct by the local secretion of several hormones or the stretched stimulation.