Induction of Odontoblast from Stromal Cell in Dental Pulp and Bone Marrow

仅次于器官移植的医学技术是组织工程。硬组织骨和牙齿的组织工程研究被认为是牙科学研究者的使命。因此，我们正在研究牙髓和骨髓基质细胞诱导成骨细胞和成牙细胞分化，以进行临床骨牙再生。在对牙髓的研究中，我们发现H-2k^b-tsA58小鼠永生牙髓细胞表达DSPP基因，具有碱性磷酸酶活性，形成羟基磷灰石，更能形成软骨组织。结果表明，成年小鼠牙髓中存在分化为成牙髓细胞和成软骨细胞的多电位间质细胞。然后，由于培养细胞形成的细胞外基质对细胞分化具有重要作用，我们在成骨细胞MC3T3-E1上对骨外基质的形成机制进行了分析。本实验结果阐明了钛与体外培养成骨样细胞间细胞外基质形成的过程。此外，我们还通过分离细胞与细胞外基质的方法，成功地开发了与细胞外基质结合的组织集成生物材料。目前，骨细胞外基质在泰坦板上诱导骨髓基质细胞成骨的研究正在进行中。在本报告中，下列五章详细说明了这些结果。第1章生物集成材料与组织工程——贴近细胞的生物集成材料。牙髓细胞具有向成牙髓细胞和成软骨细胞分化的多种潜能。第3章MC3T3-E1细胞在骨片上培养的作用和命运。第四章细胞外基质中胶原纤维的形成始于一个球形体。第五章完整细胞外基质作为生物材料和组织工程的应用。少

The attracted medical technology next to organ transplant is tissue engineering. It is thought to be mission entrusted to the researcher in dental science that tissue engineering of bone and tooth in hard tissue is developed. Therefore, we are studying on induction of differentiation for osteoblast and odontoblast from stromal cell in dental pulp and bone marrow, in order to carry out clinical regeneration of bone and tooth. On the study of dental pulp, we found that immortal pulp cell of H-2k^b-tsA58 mouse expressed DSPP gene, had alkaline phosphatase activity and formed hydroxyapatite, still more formed cartilage tissue in pellet culture. These results indicated that multipotential stromal cell which can differentiate to odontoblast and chondroblast presented in dental pulp of adult mouse. Then, as extracellular matrix formed by cultured cell was important for cell differentiation, the mechanism of formation of bone extra cellular matrix was analyzed on osteoblastic MC3T3-E1 cell cul … More tured on bone slice and titanium.The course of extracellular matrix formation between titanium and cultured osteoblast-like cell was clarified by the present results. Moreover, we succeeded in development of tissue integrated biomaterial combined with extracellular matrix by the method of separating cell from extracellular matrix.. Now, the study on induction of osteoblast from stromal cell in bone marrow on the titan plate with bone extracellular matrix was currying out. In the present reports, these results were explained in details in following five chapters.Chapter 1 Bio-integrated materials and tissue engineering-Bio-integrated material tender to cell.Chapter 2 A dental pulp cell has multi-potential for differentiation to odontoblast and chondroblast.Chapter 3 The role and fate of MC3T3-E1 cell cultured on bone slice.Chapter 4 Collagen fiber formation in extracellular matrix starts as a spherical body.Chapter 5 Intact extracellular matrix for use as biomaterial and in tissue engineering. Less