Functional and morphological analysis orme main excretory auct epithelium of the salivary gland. VI. Secretory ability of the tuft cell

1. 微绒毛表面与簇细胞的管泡系统表现出SBA（大豆凝集素）反应性，表明两者之间存在一定的关系。3.对簇状细胞进行三维重建，揭示了簇状细胞与低胚芽细胞之间的关系，以及由复杂而一致的小泡和小管网络分别组成的管泡系统，提示了膜的快速表面积变化。用光镜和电子显微镜放射自显影法研究了主耳管簇状细胞分泌糖蛋白的能力。将糖蛋白前体3h -半乳糖静脉注射到大鼠体内。于注射^ 3h -半乳糖后5min、20min、2h、6h、24h处死动物。结果表明，簇状细胞很容易将^ 3h -半乳糖合并到膜糖蛋白中，这些糖蛋白被合并到质膜中，并且没有能力将糖蛋白分泌到唾液中。

1. The surface of microvilli and the tubulovesicular system of the tuft cell exhibited the SBA (Soybean agglutinin) reactivity, suggesting a elationship between them.2. 3-D reconstruction of the tuft cell reveald the hypolemmal terminal-tuft cell relationship and that tubulovesicular system composed of the complex and coherent vesicles and small network of tubules separated respectively, suggesting the stock of membrane for rapid change of the surface area.3. The ability to secrete the glycoprotein by the tuft cells in the main exc etory duct was investigated with light and electron microscope radioautographic study. The glycoprotein precursor 3H-galactose was injected intravenously into rats. At 5min, 20min, 2h,6h, 24h after injection of ^3H-galactose, animals were sacrificed. The results indicated that the tuft cells readily incorporate ^3H-galactose into the membrane glycoproteins, which are incorporated into the Plasma membrane and that have no ability to secrete theglycoprotein into the saliva.