A study on the detection of periodontal pathogens from prepubertal periodontitis patients by PCR

本研究的目的是用聚合酶链式反应(PCR)检测104名儿童牙菌斑样本中10种可能的牙周病原体。所有受试者的年龄范围为2-12岁。在乳牙和混合牙列中均检出伴生放线菌和牙龈假单胞菌。放线菌伴生菌在健康人群中的患病率为4.8%，其中牙周炎和牙周炎的患病率分别为6.8%和20.0%；我们用牙刷进行的调查表明，在健康儿童的口腔中很少出现伴生放线菌和牙龈假单胞菌。健康体检者、牙周炎患者和牙周炎患者的痰假单胞菌感染率分别为48.0%、36.5%和25.0%，白色毛念珠菌分别为100%、89.4%和50.0%，牙周炎患者分别为96.0%、84.7%和75.0%。我们的结果表明，Sputigena中度流行，而在受试儿童的口腔中普遍检测到C.ochracea和C.givalis，这表明所有这些物种都是在早期形成的。健康人群中福赛斯球虫感染率为33.3%，牙周炎患者为63.9%，牙周炎患者为58.3%，牙周炎患者为18.1%，牙周炎患者为16.7%，牙周炎患者为0.0%。所有受试者的直肠肌患病率均为100%。我们的调查表明齿弓形虫可能与牙周病有更多的关联，连翘尾丝虫是中等流行的，而直头肌是儿童口腔中最常见的，可以在他们的早期建立。

The purpose of this study was to detect the presence of 10 putative periodontal pathogens in plaque samples from 104 children, collected from their toothbrushes using a polymerase chain reaction (PCR). The age range of all subjects was 2-12 years.1. Both A.actinomycetemcomitans and P.gingivalis were detected in the primary and mixed dentition. The prevalence of A.actinomycetemcomitans in healthy subjects was 4.8%, and those with gingivitis and periodontitis was 6.8% and 20.0% respectively, while the prevalence of P.gingivalis was 4.8% in healthy subjects, and 9.6% and 20.0% in those with gingivitis and periodontitis, respectively. Our survey, using a toothbrush, indicated that A.actinomycetemcomitans and P.gingivalis are rarely present in oral cavities of healthy children.2. C.sputigena prevalence in healthy, gingivitis, and periodontitis subjects was 48.0%, 36.5% and 25.0%, respectively, that for C.ochracea was 100%, 89.4%, and 50.0%, respectively, and that for C.gingivalis was 96.0%, 84.7%, and 75.0%, respectively. Our results showed that C.sputigena was moderately prevalent, whereas C.ochracea and C.gingivalis were commonly detected in the oral cavities of the tested children, suggesting that all of these species become established in the early years.3. The prevalence of B.forsythus in healthy subjects was 33.3%, those with gingivitis was 63.9%, and those with periodontitis was 58.3%, while the prevalence of T.denticola was 0.0% in healthy subjects, and 18.1% and 16.7% in those with gingivitis and periodontitis, respectively. The prevalence of C.rectus was 100% in all subjects. Our survey indicated that T.denticola could be more associated with periodontal diseases and B.forsythus has a moderately prevalence, while C.rectus is most commonly present in the oral cavity of children and could be established in their early years.