Enzymatic Treatment of Textile Dysing Effluents

报道了胆红素氧化酶（BO）对各种染料废水的生物降解性及处理效果。采用土壤灌注法首次研究了酞菁铜（CP）的生物降解。CP水溶液分别在含有原生土壤和灭菌过的田间土壤的柱间循环。原生土柱的od值（λ max=622 nm）和溶液中总铜浓度在2周内迅速下降到几乎为零，而灭菌土柱则没有显著下降。在含有0.1% CP作为唯一碳源的琼脂培养基上生长的真菌经过292天的循环从前柱中分离出来。根据菌体形态特征鉴定该真菌为疣状密孢子菌。根据Murao和Tanaka的研究（农业生物化学）。[j], 1981,45,2383)，从土壤中分离的疣状黑僵菌成功生成BO。在pH值为5 ~ 7、温度为32 ~ 37℃的条件下，BO对CP有较好的脱色效果。用BO对21种染料（酸性蓝25、40、41、45、80、138、酸性绿25、27、活性蓝2、4、5、19、活性黑5、直接蓝6、15、106、直接绿59、直接黑22、51、直接紫48、直接红2）的水溶液进行脱色。用高效液相色谱法分析脱色液的吸收光谱，用光电二极管阵列分析检测其中间体。

The biodegradability of various kinds of dyes and their effluent treatment by use of an enzyme, Bilirubin oxidase (BO) are reported. Biodegradation of copper phthalocyanine (CP) was first examined by using the soil perfusion method. An aqueous CP solution was circulated through columns containing native and sterilized field soils, respectively. A rapid decrease to almost zero in both O.D.(λ max=622 nm) and total copper concentration in the solution was observed within 2 weeks in the native soil column, but no significant decrease in the sterilized soil column. A fungus growing on an agar medium containing 0.1% CP as a sole carbon source was isolated from the former column after 292 days of circulation. The fungus was identified as Myrothecium verrucaria according to the characteristic morphology of its phyalides. According to Murao and Tanaka (Agri.Biol.Chem., 1981, 45, 2383), Myrothecium verrucaria isolated from soil generates BO successfully. CP was efficiently decolorized by the BO under the optimum conditions of pH 5-7 and of temperatures 32-37℃. Aqueous solutions of 21 dyes (C.I.Acid Blue 25, 40, 41, 45, 80, 138, Acid Green 25, 27, Reactive Blue 2, 4, 5, 19, Reactive Black 5, Direct Blue 6, 15, 106, Direct Green 59, Direct Black 22, 51, Direct Violet 48, Direct Red 2) were decolorized by BO.The absorption spectrum of decolorized solutions were analysed by HPLC, and their intermediats were detected by the photodiode array analysis.