The development of materials for foods and drugs by bio-power of marine microorganisms

我们建立了一个海洋微生物文库，以寻找能够产生对开发新食品或药物有用的材料的细菌。该微生物文库为我们提供了以下新结果。首先，我们发现了两种海洋细菌菌株，它们显示出抑制黑色素生成的能力，与0。用25mm抗坏血酸筛选548株海洋细菌。细菌分泌的抑制剂是耐热的，没有还原活性。然后，通过筛选2480株海洋细菌，我们也发现了24株海洋细菌具有抑制结肠癌细胞生长的能力。此外，我们发现海藻酸盐的成分之一甘露酸盐聚合物在x射线照射后抑制了具有野生型p53基因的胶质母细胞瘤细胞的运动。此外，我们还发现半乳糖醛酸可以刺激marina查顿菌和赤谷异信号菌产生更多的O_2-，这表明半乳糖醛酸与鞭毛细胞表面特定位点的结合可能会导致O_2-的产生增加。最后，我们建立了对海洋资源中内分泌干扰物超敏感的体外和体内筛选系统。

We constructed a marine microorganism library to find the bacteria that produce materials useful for developing new foods or drugs. This microorganism library provides us the following new results. First, we found two strains of marine bacteria showing the ability to inhibit melanogenesis that is comparable to that of 0. 25 mM ascorbic acid by screening 548 strains of marine bacteria. The bacteria-secreted inhibitors are heat-resistant and show no reductive activity. Then, we also found 24 strains of marine bacteria showing the ability to inhibit the growth of colon cancer cells by screening 2,480 strains of marine bacteria. In addition, we found that mannuronate polymer, one of components of alginate, suppressed the motility of glioblastoma cells having wild-type p53 gene after X-irradiation. Furthermore, we found that galacturonic acid stimulated Chattonella marina and Heterosigna akashiwo to generate increased amounts of O_2-, suggesting that the binding of galacturonic acid to specific sites on the flagellate cell surface may induce the increase of O_2- production. Finally, we established in vitro and in vivo screening systems that were hypersensitive to detect endocrine desruptors in the marine resources.