Intensified process coupling reaction with separation using enzymesfunctionalized membranes for the recovery of bIomolecules from microalgae

该高级项目的目的是开发一种生物催化膜反应器，以有效地从微藻中回收有价值的生物分子。Premium涉及过程强化，并依赖于多学科方法：1)过程和生物过程工程与生物质预处理，一种结合酶反应和膜过滤的新型反应器的开发和建模；2)聚合物科学，与特殊膜的开发和通过电子束处理使其酶功能化；3)材料科学，与改进的低温电子显微镜方法的开发，以获得关于功能化膜(酶的定位和固定化产量)及其对污染的影响的必要知识，以补充创新的过程开发和建模。为了使这个项目取得成功，有必要结合GEPEA(FR)实验室在过程工程领域的专业知识，该实验室专门从事微藻生物资源的培养和生物精炼，IOM(GER)掌握膜的制造和功能化，并拥有用于共价功能化膜功能化的独特专业知识和设备，以及IMN(FR)，它开发了创新的高分辨率电子显微镜方法，在天然(水合)条件下表征功能化多孔介质和受污染的膜，这是目前尚不存在的。

The aim of the PREMIUM project is to develop a biocatalytic membrane reactor for the efficient recovery of valuable biomolecules from microalgae. PREMIUM deals with process intensification and relies on a multidisciplinary approach: 1) process and bioprocess engineering with biomass pretreatment, the development and modeling of a new reactor coupling enzymatic reaction and membrane filtration; 2) polymer sciences with the development of special membranes and their enzyme functionalization by electron beam treatment; 3) Material sciences with the development of adapted cryo-electron microscopy methods to gain the necessary knowledge about the functionalized membranes (localization of enzymes and immobilization yields) and their effects on fouling to complement innovative process development and modeling. To make this project a success, it is necessary to combine the expertise of the GEPEA (FR) laboratory in the field of process engineering, specialized in the cultivation of microalgal bioresources and biorefining, the IOM (GER), which masters the fabrication and functionalization of membranes and has unique expertise and equipment for the functionalization of membranes with covalent functionalization, and the IMN (FR), which has developed innovative, high-resolution electron microscopy methods for characterizing functionalized porous media and fouled membranes under their native (hydrated) conditions, that do not currently exist.