CHARACTERIZATION OF DISSOLVED ORGANIC MATTER BY THREE DIMENSIONAL EXCITATION EMISSION MATRIX SPECTROSCOPY

利用三维激发发射矩阵对湖泊、河流、二级污水等多种样品进行了荧光光谱表征。我们的结果表明，溶解有机质可以分为三个不同的类别。组1的区别在于激发波长为270 nm、发射波长为435 nm的黄腐酸类荧光(Ex.495 nm/Em。435 nm)。利用这个相对荧光强度可以计算出黄腐酸类物质的浓度。Grplum2的区别在于蛋白质类型的荧光(Ex.280 nm/Em。340 nm)。该物质由二级出水的蛋白质和藻类释放的蛋白质组成。组3的区别在于二级流出物中的有机物引起的荧光(例如。495 nm/Em。515 nm)。这种物质是疏水的，不能生物降解。激发波长为495 nm、发射波长为515 nm的相对荧光强度可以作为二次流出物影响的一个指标。此外，非离子表面活性剂如壬基酚聚氧乙烯醚和荧光增白剂(4，4‘-双[2-磺基苯乙基]联苯)也可以通过Ex的荧光来表征。230 nm/Em。295±5 nm和Ex.350 nm/Em。分别为435 nm和435 nm。这些物质可以用反相高效液相色谱和荧光激发发射矩阵法相结合的方法进行定量分析。阐明了这些物质在污水处理系统中的行为。

Various samples from lakes, rivers, secondly effluents and so on were characterized by fluorescence spectroscopy using three dimensional excitation-emission matrix. Our results suggest that dissolved organic matter can be categorized into three distinct groups. Group1 is distinguished by a fulvic acid-type fluorescence of excitation at 270nm and emission at 435nm (Ex. 495nm/Em. 435nm). Fulvic acid-type substances concentration can be calculated using this relative fluorescence intensity. Grpup2 is distinguished by a protein-type fluorescence (Ex. 280nm/Em. 340nm). This substance consisted of protein from secondly effluent and that released by algae. Group3 is distinguished by a fluorescence caused by organic matter in secondly effluent (Ex. 495nm/Em. 515nm). This substance was hydrophobic and not biodegradable. The relative fluorescence intensity in excitation 495nm and emission 515nm may be an indicator of the influence of secondly effluent.Moreover, the non-ionic surfactants such as nonylphenol ethoxylates and fluorescent whitening agents (4,4'-bis[2-sulfostyryl] biphenil) can be characterized by the fluorescence of Ex. 230nm/Em. 295± 5nm and Ex. 350nm/Em. 435nm respectively. These substances can be quantitatively analyzed by using both the opposite phase HPLC and the fluorescence excitation and emission matrix method in combination. The behaviors of these substances during sewage treatment system were clarified.