DEUTERIUM OXIDE ON FLUORESCENCE INTENSITY & LIFETIME OF DNA INTERCALATING PROBES

光谱分析和常规及相敏流动 用流式细胞术比较PI和EB荧光的变化 与DNA和固定的中国仓鼠卵巢结合的强度和寿命 (CHO)D2 O与磷酸盐缓冲盐水存在下的细胞 （PBS）。 PI和EB的荧光强度增强两倍 与D2 O中固定的CHO细胞结合，并增加5 ns 在D_2O中的PI和EB荧光寿命。 我们最近使用了D2 O 在EB与人二倍体成纤维细胞结合的寿命研究中， 位于G1/S相变点。 结果表明 在进入S期结构期间改变染色质状态 导致荧光强度和荧光 DNA嵌入EB的寿命。

Spectroscopic analysis and conventional and phase-sensitive flow cytometry were used to compare changes in PI and EB fluorescence intensity and lifetime bound to DNA and fixed Chinese hamster ovary (CHO) cells in the presence of D2O versus phosphate buffered saline (PBS). A two-fold enhancement of fluorescence intensity of PI and EB bound to fixed-CHO cells in D2O was noted as well as a 5 ns increase in PI and EB fluorescence lifetimes in D2O. We have recently used D2O in studies on the lifetime of EB bound to human diploid fibroblasts located at the G1/S phase transition point. Results indicate that alterations chromatin states during the entry into S phase structures lead to alterations in the fluorescence intensity and fluorescence lifetime of the DNA-intercalated EB.