Investigation of culturial physiology of tomato by analysis of phytohormone

对3个月龄番茄植株的内源吲哚-3-乙酸(IAA)进行了液-质联用法测定，用荧光检测法测定了植株的扩散态含量，用放射性IAA测定了植株的极性传递活性。在茎的上部和下部，质外体(AP，细胞外汁液)的IAA浓度都高于共生体(SP，细胞内的汁液)，这表明对AP进行IAA分析是重要的。年轻叶片比老叶输出更多的可扩散的IAA。主根和茎中的IAA浓度基本持平。分析了单性结实番茄无核果实中IAA、赤霉酸(GAL)、反式玉米素(Z)和反式玉米素核苷(ZR)的含量。细胞扩张期中间直径6 cm的阶段称为扩张期，细胞扩张期末期的直径为8 cm的阶段称为MG期。果皮和果壁的IAA浓度在MG期高于膨大期。在分隔区中，无论是膨胀期还是MG期，SP的IAA浓度都高于AP。在Z浓度方面，膨大期果皮的SP含量最高，是AP的2.7倍，而AP和SP的果皮中SP含量相同。膨大期子房组织中ZR含量最高。结果表明，合成的部位可能是IAA和Z的SP和ZR的小室。

As to indole-3-acetic acid(IAA), endogenous levels by liquid chromatography-mass spectrometry, diffusible levels by fluorometric detection and polar transport activity by radioactive IAA were determined in 3-month-old tomato plants. IAA concentration in the apoplast(AP, sap outside a cell) was higher than those in the symplast (SP, sap within a cell) in both upper and lower parts of stems, showing that IAA analysis of AP is important. Younger leaves exported much more diffusible IAA than older leaves. IAA concentration in the main roots was almost at the same level as in the stems. It is suggested that at sampling, the transport capacity of IAA is probably the limiting factor for IAA transport in stems, and the amount of polar IAA transport might be only 19% of the endogenous IAA amount in stems.The levels of IAA, gibberellic acid1 (GAl), trans-zeatin (Z) and trans-zeatin riboside(ZR) in seedless fruits of parthenocarpic tomato were analysed. Stage in 6cm diameter corresponding the middle of cell expansion phase were referred to expansion-stage, and stage in 8cm diameter corresponding the last of cell expansion phase were referred to MG-stage. IAA concentrations of the pericarps and partitions were higher in MG stage than in expansion-stage. In the partitions, IAA concentrations of SP were higher than those of AP in both expansion-and MG-stage. As to Z concentration, the SP of the partitions in expansion-stage had the highest and was 2.7 times than the AP, while in the pericarps were the same level in AP and SP. The ZR concentration in locule tissues in expansion-stage was the highest of all parts. The results suggest that the sites of synthesis may be the SP of partitions for IAA and Z, and locules for ZR.