Investigation of physiological mechanism and development of applied technique for horticultural crop by analysis of property of transport and metabolism of auxin

生长素转运代谢特性分析，探讨园艺作物生理机制及应用技术开发

• 批准号: 16580015
• 负责人: KOJIMA Kiyohide
• 金额: $ 2.37万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580015/
• 关键词: Indole-acetic acid; Auxin; Horticultural crop

The analysis method of indole-3-acetic using gas chromatography mass spectrometry (GC-MS) was confirmed and using this system the analysis of indole-3-acetic was performed. As to the metabolic rate of indole-3-acetic acid, younger fruits in cucumber was faster than older fruits.To investigate the relationship between the flow of indole-3-acetic and physiology of tree vigor, the level of diffusible indole-3-acetic from water sprout (shoot) was determined using three cultivers of Japanese pear (Housui, Akizuki, Sinsui). In middle of June, the stage of end of branch elongation, the level of diffusible indole-3-acetic from water sprout of three cultivers was related to empirical knowledge. Thus, it was suggested that tree vigor could be evaluated by the determination of the level of diffusible indole-3-acetic in this stage.To confirm the production sites of indole-3-acetic acid responsible for rapid elongation of the floral stalk of tulip, the diffusible indole-3-acetic acid from different plant organs was studied. During the period of rapid elongation of the floral stalk, the levels of diffusible indole-3-acetic acid exuded from different organs were internodes>floral organs>leaves. The amount of the indole-3-acetic acid diffused from the internodes and flowers differed and reached its peak at different time. The results obtained suggest that the top internode is probably the most important production site of indole-3-acetic acid accountable for the elongation of the flower stalk. Elongation of the 1-3internodes is probably also under the control of indole-3-acetic acid produced in themselves.

确定了吲哚-3-乙酸的气相色谱-质谱联用分析方法，并利用该系统对吲哚-3-乙酸进行了分析。以日本梨3个品种（丰水、秋月、新水）为试材，测定了黄瓜幼果中吲哚乙酸的代谢速率，探讨吲哚乙酸的流动与树体活力生理的关系。6月中旬，分支伸长末期，3个品种水芽中的吲哚-3-乙酸含量与经验知识有关。为了确定郁金香花柄快速伸长过程中吲哚乙酸的产生部位，对郁金香不同器官中吲哚乙酸的含量进行了研究。在花柄快速伸长期间，各器官分泌的吲哚乙酸含量为节间>花器官>叶片。吲哚-3-乙酸从节间和花中扩散的量不同，在不同的时间达到高峰。结果表明，顶节间可能是吲哚-3-乙酸产生的最重要的网站负责花柄的伸长。第1- 3节间的伸长可能也受自身产生的吲哚乙酸的控制。

项目成果

Effects of rootstock on flower bud formation in shootsof pear

砧木对梨芽花芽形成的影响

• 发表时间: 2004
• 期刊: Bull.Fac.Agric.Niigata Univ. 59(2)
• 作者: 吉岡俊人;藤郷 誠;吉岡俊人;Toshihito YOSHIOKA;吉岡俊人;吉岡俊人;Toshihito YOSHIOKA;知野秀次;知野秀次;Shuji Chino;知野秀次;小式澤一博;K.Kojima;Kiyohide Kojima;K.Kojima;知野秀次;太田祐樹;Shuji Chino;Yuuki Ohta;Shuji Chino
• 通讯作者: Shuji Chino

GA_3 overcomes bud deterioration in tulip (Tulipa gesneriana L.) bulbs during dry storage by promoting endogenous IAA activity in the internodes

GA_3 通过促进节间的内源 IAA 活性来克服干燥储存期间郁金香 (Tulipa gesneriana L.) 球茎芽的退化

• 期刊: Plant Growth Regulation (印刷中)
• 作者: 吉岡俊人;藤郷 誠;吉岡俊人;Toshihito YOSHIOKA;吉岡俊人;吉岡俊人;Toshihito YOSHIOKA;知野秀次;知野秀次;Shuji Chino;知野秀次;小式澤一博;K.Kojima;Kiyohide Kojima;K.Kojima;知野秀次;太田祐樹;Shuji Chino;Yuuki Ohta;Shuji Chino;Rong-Yan Xu
• 通讯作者: Rong-Yan Xu

エチレンあるいは低温処理がセイヨウナシ'ル レクチエ'の追熟過程の果実特性におよぼす影響

乙烯或低温处理对欧洲梨Le Lectier成熟过程中果实特性的影响

• 期刊: 園芸学研究 (印刷中)
• 作者: 吉岡俊人;藤郷 誠;吉岡俊人;Toshihito YOSHIOKA;吉岡俊人;吉岡俊人;Toshihito YOSHIOKA;知野秀次;知野秀次;Shuji Chino;知野秀次;小式澤一博;K.Kojima;Kiyohide Kojima;K.Kojima;知野秀次;太田祐樹;Shuji Chino;Yuuki Ohta;Shuji Chino;Rong-Yan Xu;Rong-Yan Xu;知野秀次
• 通讯作者: 知野秀次

2種類のHPLCカラムで精製したニンニク(Allium sativum L.)のインドール-3-酢酸, アブシジン酸およびジベレリンのGC-MS分析

使用两种 HPLC 色谱柱纯化大蒜 (Allium sativum L.) 中的吲哚-3-乙酸、脱落酸和赤霉素的 GC-MS 分析

• 发表时间: 2007
• 期刊: 新潟大学農学部研究報告 59
• 作者: 太田祐樹;他;太田祐樹;R. Y. Xu;知野秀次・児島清秀・小式澤一博・太田祐樹・中島正男・大竹憲邦・大山卓爾
• 通讯作者: 知野秀次・児島清秀・小式澤一博・太田祐樹・中島正男・大竹憲邦・大山卓爾

Analysis of the diffusible IAA from horticultural crops

园艺作物中可扩散 IAA 的分析

• 发表时间: 2004
• 期刊: Bull.Fac.Agric.Niigata Univ. 56(2)
• 作者: 吉岡俊人;藤郷 誠;吉岡俊人;Toshihito YOSHIOKA;吉岡俊人;吉岡俊人;Toshihito YOSHIOKA;知野秀次;知野秀次;Shuji Chino;知野秀次;小式澤一博;K.Kojima;Kiyohide Kojima;K.Kojima;知野秀次;太田祐樹;Shuji Chino
• 通讯作者: Shuji Chino