Degradation of residual lignin in pulp and environmental pollutants by MnP-lipid

本研究研究了锰过氧化物酶（MnP）在不饱和脂肪酸（UFA）存在下的反应，即MnP-脂质过氧化体系对未漂白硫酸盐纸浆中残留木质素的降解及对环境污染物的降解。在mnp漂白体系中加入UFA可提高纸浆亮度。反应混合物中的氧化值与纸浆亮度的提高有很好的相关性。然而，脂氧化酶处理引起的脂质过氧化并没有增加牙髓的亮度。这些结果表明，MnP作用产生的Mn （III）和脂质过氧化作用协同降解木质素。此外，木质素也加速了mnp -脂质过氧化系统中的脂质过氧化2。研究了mnp -脂质过氧化体系对多芳烃（PAH）的降解。mnp -脂质过氧化体系能有效降解蒽和苯并芘。动力学研究表明UFA的过氧化比PAH的降解快得多。脂质过氧化反应包括脂质过氧化中间体的形成和自由基的生成两个反应，且前者反应更快。不同的UFA对mnp -脂质过氧化系统中多环芳烃的降解没有影响，这表明广泛的UFA对该系统起作用。尝试用mnp -脂质过氧化体系去除双酚A （BPA）和壬基酚（NP）的雌激素活性。BPA和NP的雌激素活性在6至12小时内被完全去除。延长治疗至48小时后，再也不能恢复雌激素活性。

In this study, manganese peroxidase (MnP) reaction in the presence of unsaturated fatty acid (UFA), so called MnP-lipid peroxidation system, was investigated for the degradation of the residual lignin in unbleached kraft pulp and the degradation of environmental pollutants.1. The pulp brightness increase was increase by the addition of UFA to MnP-bleaching system. The proxidation value in the reaction mixture was well collated to the increase of pulp brightness. However, lipid peroxidation derived by lipoxidase treatment did not increase pulp brightness. These results suggest that Mn (III) produced by MnP action and lipid peroxidation synergistically degrade lignin. Additionally, lignin in the pulp also accelerated the lipid peroxidation in MnP-lipid peroxidation system.2. Degradation of polyaromatic hydrocarbons (PAH) by MnP-lipid peroxidation system was investigated. Anthracene (AT) and benzopyrene were effectively degraded by MnP-lipid peroxidation system. A kinetic study indicates that peroxidation of UFA was much faster than PAH degradation. It is also suggested that lipid peroxidation reaction involves two reaction, formation of lipid-peroxide intermediates and generation of free radicals, and the former reaction is faster. Degradation of PAH was not changed in the MnP-lipid peroxidation system using different UFA, suggesting that wide range of UFA are functional for this system.3. Removal of estrogenic activity of bisphenol A (BPA) and nonylphenol (NP) by MnP-lipid peroxidation system was attempted. The estrogenic activity of BPA and NP was completely removed within a 6 to 12 hour treatment. The extended treatment to 48 h never regenerates the estrogenic activity.