Correlated Light and Electron Microscopic Study of the Plasmalemmal Undercoats

细胞骨架是原核细胞的重要组成部分，起着细胞机械支撑、胞质分裂、细胞黏附、信号传递、细胞分化和发育等作用。它们的许多功能是基于与细胞黏附结构和质膜底膜的机械和结构联系。我们用电子显微镜结合共聚焦显微镜和生物化学技术研究了肌肉细胞和神经周围细胞中的这种联系。此外，我们还利用相关的分子生物学和显微镜技术研究了嘴端决定基因OTX对脊椎动物胚胎发育过程中细胞黏附的诱导作用。我们还鉴定了一种原钙粘附素作为体细胞发生的形态调节剂。

Cytoskeletons are among the key components of prokaryotic cells playing such rolls as a cellular mechanical support as well as cytokinesis, cell adhesion, signal transmission, cell differentiation and development. Many of their functions are based on the mechanical and structural associations with cell adhesion structures and plasmalemmal undercoat. We studied such associations in muscle cells and perineurial cells by electron microscopy correlated with confocal microscopy and biochemical techniques. Additionally we investigated the induction of cell adhesion by rostral determinant gene Otx in developing vertebrate embryos by correlated molecular biology and microscopy. We also identified a protocadherin as a morphoregulator of somitogenesis.