The study on the morphogenesis of peroxisomes employing a targeting signal and its receptors

通过表达在其C-末端具有靶向信号的绿色荧光蛋白的融合蛋白（GFP-SKL）来染色过氧化物酶体，通过转染程序将其引入培养的细胞，并通过制备转基因小鼠引入小鼠。本研究的目的是以这些细胞和转基因小鼠为材料，阐明其形态发生的机制。本研究试图显示过氧化物酶体的外观在细胞分裂，胚胎发育和管理的过氧化物酶体增殖剂。转基因小鼠表达。已经研制出绿色荧光蛋白-国家重点实验室，正在进行生物化学和形态学分析。已对表达GFP-SKL的培养细胞进行了形态学分析，并即将发表。过氧化物酶体的相关课题已经发表。

Peroxisomes have been stained by expressing a fusion protein of green fluorescent protein with a targeting signal in its C-terminus (GFP-SKL), introduced to cultured cells by trasfection procedure and to mice by making a trasgenic mouse. The object of the study is to elucidate the mechanism of morphogenesis, with these cells and transgenic mouse as materials. The present study was attempted to show the appearance of peroxisomes during cell division, embryonic development and by the administration of peroxisome proliferators. A tansgenic mouse expressing. GFP-SKL has been developed and biochemical and morphological analyses are on the way. Morphological analyses in cultured cells expressing GFP-SKL have been analyzed and on the way of publication. Related subjects on peroxisomes have been published.