Association Between Serum Cytokine Concentration And Between-subject variability in Airway Obstructive Response In Welders.

本研究的目的是在控制了磁气成像（MPG）估计的累积暴露水平后，评估电弧焊工中几种类型的细胞因子血清水平与焊接烟雾肺反应的个体变异性之间和受试者内部的变异性。研究对象来自某工厂约150名主要从事电弧焊的工人。在获得每位参与者的知情同意后，我们在2000年和2001年使用高灵敏度化学发光ELISA法检测了血清中白细胞介素-4 （IL4）、白细胞介素-6 （IL6）、白细胞介素-8（11.8）和干扰素-γ (IFNγ)的水平（Satoh T. et al ., 1995）。此外，我们还测量了血清和尿液中的其他生物标志物，如尿中的8-羟基-2-脱氧鸟苷和可替宁水平。通过MPG和肺功能水平估计的累积暴露的纵向变化也进行了评估。基线测量后5年以上，所有细胞因子分布范围广，并表现出向正方向的中度或重度偏态。然而，在2000年的第一次测量和2001年的第二次测量之间，观察到每种细胞因子的显著正相关。这些结果表明，受试者体内细胞因子的变异性并不像横断面估计的受试者之间的差异那样大。因此，有可能考虑到每个工人都有自己的细胞因子参考水平。另一个有趣的结果是血清il - 4和il - 8水平显著正相关（Spearman等级相关系数=0.62）。这可能表明焊接烟雾对呼吸系统影响的共同方面。MPG测量显示，在五年的随访中，工人肺部的磁化率显著降低。据估计，平均减少66%的烟雾沉积。因此，充分调节工作环境将有助于减少接触焊接烟雾和消除已经积聚在肺部的微粒。血清细胞因子水平与累积暴露于焊接烟雾和肺功能变异性之间的关系目前正在详细研究中。少

The objective of this study was to evaluate between and within-subject variability of serum level of several types of cytokine among electric arc welders, in relation with individual variability of pulmonary response to welding fumes, after controlling for cumulative exposure levels estimated by magnetopneumography (MPG).Study subjects were derived from an occupational cohort which was consisted of about 150 workers mainly engaging in electric arc welding in a factory. After given a informed consent from each participant, we examined serum level of interleukin-4 (IL4), interleukin-6 (IL6), interleukin-8 (11.8), and interferon-γ (IFNγ) in 2000 and in 2001, utilizing high sensitivity chemiluminescence ELISA method (Satoh T. et al, 1995). In addition, we also measured several other biomarkers in serum and in urine, such as 8-hydroxy-2-deoxyguanosine and cotinine levels in urine. Longitudinal change in cumulative exposure estimate by MPG and pulmonary function levels were also evaluated fi … More ve years after the baseline measurementsAll cytokines distributed in wide ranges and showed moderate or severe skewness towards positive direction. However, significantly positive association was observed between the first measurements in 2000 and the second ones in 2001 in each cytokine. These results suggest that withinsubject variability of cytokines is not so large as expected from cross-sectionally estimated betweensubject variation. Thus, it might be possible to consider that each individual worker has his own reference level of cytokines. Another interesting result was significant positive association between serum IL4 and IL8 levels (Spearman s rank correlation coefficient=0.62). It might indicate common aspects of the influence of welding fumes on respiratory system. MPG measurements showed marked reduction in magnetizability of the workers lung in a five-year follow-up. It was estimated as 66% decrease in deposition of fumes on average. Therefore, sufficient regulation of the working environment will be helpful in reducing the exposure to welding fumes and eliminating particulates already accumulated in the lungs. The association of serum cytokine levels with cumulative exposure to welding fumes and pulmonary function variability is now under consideration in detail. Less