Study on surfactant-associated protein as a biomarker in the environmental pollutant exposure

本研究旨在探讨肺表面活性物质相关蛋白(SP-A、SP-D、KL-6)是否为矿物粉尘接触和纤维化进展过程中间质改变的血清生物标志物。此外，还探讨是否将表面活性物质相关蛋白作为环境污染物暴露的血清生物标志物。采用抗中性粒细胞胞浆抗体(ANCA)和细胞因子对尘肺患者、间质性肺疾病石棉肺患者和接尘工人进行了表面活性物质相关蛋白的检测。此外，表面活性物质相关蛋白的测定作为评估环境污染物暴露对健康的不良影响的血清生物标志物的有效性被检验。特发性肺纤维化在接尘工人和尘肺患者中都有很高的发生率。在这些…中表面活性物质相关蛋白水平升高的病例较多，提示与职业性粉尘接触有关。ANCA的产生和炎性细胞因子的增加可能激活了中性粒细胞，肺组织和血管的炎症通过蛋白水解酶和活性氧的产生而持续，最终可能导致间质性肺炎的发生。因此，尘肺患者和接尘工人肺表面活性物质相关蛋白的增加是有用的血清生物标志物，反映了职业接尘引起的间质性改变的过程。在焚烧炉周围居民的健康调查中，关于作为评估环境污染物暴露对健康不利影响的血清生物标志物的有用性，尚不清楚，似乎有必要在未来进行进一步的检查。较少

The purpose of this study was to investigate whether surfactant-associated proteins (SP-A, SP-D, KL-6) are serum biomarker of interstitial change with the mineral dust exposure and fibrosis progress. In addition, it was to investigate whether it evaluates surfactant-associated protein as serum biomarker in the environmental pollutant exposure. In pneumoconiosis patients, asbestosis patients who is the interstitial lung disease and dust exposure workers, we carried out the surfactant-associated proteins measurement with anti-neutrophils cytoplasm antibody (ANCA) and cytokines. And, the usefulness of the surfactant-associated proteins measurement were examined as a serum biomarker for evaluating the adverse effect to the health which accompanies the environmental pollutant exposure in the general inhabitants who lives in the neighbourhood of incineration facility.Idiopathic pulmonary fibrosis was seen to both of dust exposure workers and pneumoconiosis patients at the high rate. In these … More cases, that the increase in surfactant-associated protein level was observed indicated the relation with the occupational dust exposure. ANCA production and increase in the inflammatory cytokines were supposed that it activated neutrophils and that the inflammation of pulmonary tissue and blood vessel continues by the production of proteolytic enzymes and active oxygen, and that then, it may finally induce it to the development of the interstitial pneumonia.Therefore, the increase in surfactant-associated proteins was observed to pneumoconiosis patients and dust exposure workers, and it was indicated that it was useful as serum biomarker which reflects the process for the interstitial change with the occupational dust exposure. In the health survey in the inhabitants around the incinerator, on the usefulness as a serum biomarker for evaluating adverse effect to the health with the environmental pollutant exposure, it was not clear, and further examination would seem to be necessary in future. Less