CELL-TO-CELL INTERACTION IN THE MARGINALSINUS OF MESENTERIC LYMPH NODE

1)大鼠肠系膜淋巴结微淋巴管的分布和淋巴细胞的迁移。血中标记淋巴细胞优先与Peyer‘s斑状小静脉黏附。淋巴细胞从小静脉移行，进入Peyer‘s小结的传出淋巴管，再流入淋巴结。淋巴细胞被滞留在边缘窦中，边缘窦有足够的表面供淋巴细胞聚集。结果表明，归巢于肠系膜淋巴结的淋巴细胞主要通过传入淋巴管。2)巨噬细胞在肠系膜淋巴结内迁移的情况。外照下可见颗粒膜巨噬细胞数量在注射后12小时内增加，然后下降。一些富含颗粒的巨噬细胞进入边缘窦，并迅速流向淋巴结门。在冷冻切片中，富含颗粒的巨噬细胞横跨…随着时间的推移，更多的细胞通过皮质向髓质迁移。异物吞噬后，ED3阳性巨噬细胞通过边缘窦或皮质向髓质迁移，寻找免疫增殖性微环境。3)早期淋巴结转移的实验动物模型及抗原提呈细胞的反应。在注射后6小时，流入边缘窦的癌细胞数量达到最大。注射后4d，边缘窦内充满癌细胞并扩张。在癌细胞衬里下面，有ED3阳性的巨噬细胞聚集.他们加入了凋亡的癌细胞。树突状细胞位于癌旁皮质，不聚集，但分布于癌细胞团的边缘。在缺乏巨噬细胞的淋巴结中，癌细胞很早就侵入了副大脑皮层。4)树突状细胞从肠道向淋巴结迁移，标记的树突状细胞于注射至盲肠粘膜下层后6小时即可迁移至肠系膜淋巴结，48小时达高峰，72小时后下降。体外细胞因子刺激上调树突状细胞MHC-II类分子的表达，导致早期向淋巴结转移。较少

1) Distribution of lymphatic microvessels and migration of lymphocyte in the mesenteric lymph node of ratLabeled lymphocytes in blood adhered to Peyer's patch venules preferentially. The lymphocytes transmigrated from the venules and entered efferent lymphatics in Peyer's patches, and the flowed into the lymph node. The lymphocytes were arrested in the marginal sinus, which has huge surface sufficient for lymphocytes pooling. It is indicated that lymphocytes homing to mesenteric lymph nodes predominantly passes through afferent lymphatics.2) Visualization of macrophage migration in mesenteric lymph nodeFluorescent particles were taken up by EDS positive macrophages beneath the marginal sinus. The number of the particleladen macrophages seen by epi-illumination increased up to 12 hr after injection, and then declined. Some particle-laden macrophages entered the marginal sinus and flowed rapidly toward the hilus of the lymph node. In cryostat sections, the particle-laden macrophages tran … More smigrated through the cortex toward the medulla in course of time. Upon phagocytosis of foreign bodies, the ED3 positive macrophages migrate toward the medulla through the marginal sinus or cortex to find immunoproliferative microenvironment.3) Experimental animal model of early lymph node metastasis and response of antigen presenting cells.Fluorescence labeled cancer cells (AH 130 ascites hepatoma cells) were arrested in the marginal sinus of the meso-cecum lymph node. The number of cancer cells, flowed into the marginal sinus, reached the maximum 6 hours after injection. The marginal sinus was filled with cancer cells and expanded 4days after injection. Beneath the cancer cell linings, ED 3 positive macrophages have accumulated. They incorporated apoptotic cancer cells. The dendritic cell, resided in the paracortex, did not accumulate, but distributed in small number in the rim of the cancer cell cluster. In lymph nodes depleted of macrophages, the cancer cells invaded early to the paracortex. Macrophages beneath the marginal sinus play a role in the first line defense against cancer proliferation and invasion.4) Migration of dendritic cells from the gut to lymph node.Labeled dendritic cells were seen to migrate to the mesenteric lymph node 6 hours after injection to the cecum submucosa, and reached the maximum in number at 48 hours, and then declined at 72 hours. Cytokines stimulation in vitro upregulated the expression of MHC class II of dendritic cells, resulting in early transfer to the lymph node. Less