Blood flow influences vascular growth during tumor angiogenesis

我们观察了体内生长的肿瘤血管，以研究血流与血管扩大的关系。将乳腺癌移植到Fisher-344大鼠的背侧胫骨透明腔内。显微镜下观察并录像6h血管生长情况。每30min拍摄并追踪血管网络。以确定随着时间的推移发生的变化。肿瘤切片用Masson‘s三色染色和抗-8因子相关抗原染色。肿瘤的生长速度足够快，每小时都能看到差异。高血流量的血管在几个小时内直径增加，并从这些血管形成新的分支。相反，没有增加血流量的血管直径没有变化。肿瘤周围间质内的血管被抗8相关抗原染色阳性的内皮所覆盖。肿瘤内血管内有极罕见的内皮细胞，可通过Masson‘s三色或抗8相关抗原染色发现。总而言之，血流量增加可能会导致血管扩张，一些原始血管似乎缺乏内皮。

We observed growing tumor vessels in vivo to study the relationship between blood flow and vascular enlargement. Mammary adenocarcinoma was implanted into Fisher-344 rat with dorsal shin-fold transparent chambers. Vascular growth was observed and recorded on videotape through a microscope for 6h. Vascular networks were photographed and traced every 30min. To identify changes over time. Tumor sections were stained with Masson's trichrome and anti-Factor-8 related antigen. Tumor growth was rapid enough for differences to be seen each hour. Vessels with a high blood flow showed an increase in diameter within a few hours and new branches formed from these vessels. In contrast, vessels without an increase in blood flow showed no change in diameter. Vessels within the interstitium surrounding the tumor were lined by endothelium that was positive for anti-8 related antigen staining. Vessels in the tumor had extremely rare endothelial cells detectable by masson's trichrome or anti-8 related antigen staining. In conclusion, increased blood flow may cause vascular enlargement and some primitive vessels seem to lack endothelium.