Artificial Peritoneum for Cancer Research and Surgical Treatment

腹膜是一种重要的生理组织，是腹膜腔与全身循环系统之间的屏障。在本研究中，我们重建了由间皮细胞单层和结缔组织组成的人工腹膜组织。我们还利用大鼠人工腹膜移植模型建立了人工腹膜的体内模型。我们的结果可能提供一个新的有用的腹膜模型，我们可以评估许多与腹膜有关的疾病的病理生理。光镜和电镜研究证实，这种人工腹膜组织与体内组织的形态特征非常相似。该组织表面覆盖间皮单层，细胞角蛋白CAM5.2染色阳性。虽然我们尝试将IFN-_γ基因编码质粒转染到人工腹膜，但目前尚未发现可检测到的IFN-_γ。在体内模型中，我们用WKA大鼠制作人工腹膜，移植到同基因大鼠腹膜缺损处。移植腹膜与周围腹膜适应良好，宿主炎性细胞反应小，粘连小。组织学检查发现腹膜有微血管，提示移植腹膜诱导血管生成。总的来说，我们的人工腹膜提供了一种新的腹膜疾病的实验模型，如恶性或感染性腹膜炎。此外，我们的人工腹膜组织可能成为外科腹膜缺损的可移植生物工程材料。

Peritoneum is an physiologically important tissue which serve as barrier between peritoneal cavity and general circulation system. In the present study, we have reconstituted artificial peritoneal tissue which consists of mesothelial cell monolayer and connective tissue. We also established an in vivo model of artificial peritoneum using rat artificial peritoneum transplantation model. Our result may provide a new useful peritoneum model in which we can assess pathophysiology of many disease related to peritoneum. Light and elecron microscopy studies have confirmed this artificial peritoneal tissue closely resembled the morphological properties of in vivo tissue. Surface of this tissue was covered with mesothelial monolayer that was stained positive for cytokeratin CAM5.2. Although we have tried to transfect IFN-_γ gene-coding plasmid to the artificial peritoneum, no detectable amount of IFN-_γ have been found so far. For in vivo model, we have made artificial peritoneum from WKA rat and transplanted it to peritoneal defect of the syngenic rat. The transplanted peritoneum was well adapted to the surrounding peritoneum with minor response of host inflammatory cells and little adhesion. In the histological examination, the peritoneum had microvessel suggesting that the transplanted peritoneum induced angiogenesis. Collectively, our artificial peritoneum provide a novel experimental model of peritoneal disease such as malignant or infectious peritonitis. Furthermore our artificial peritoneal tissue may become a transplantable bio-engineered material for surgical peritoneal defect.