Enhancement of intestinal adaptation by epidermal growth factor in the rat small bowel transplantation

已有报道表明，在大鼠小肠移植中，表皮生长因子(EGF)在移植物适应中起着重要作用。然而，除了葡萄糖吸收外，EGF对移植肠的各种生物学功能的影响知之甚少。移植动物接受EGF或生理盐水治疗一周。除葡萄糖吸收和肠道组织学检查外，还观察了EGF对二糖酶活性和二肽转运体诱导的影响。EGF处理组的绒毛高度和隐窝深度显著高于EGF未处理组。EGF显著增加移植动物对葡萄糖的吸收和钠葡萄糖共转运体(SGLT1)的诱导。EGF还能促进麦芽糖酶和蔗糖酶等二糖酶的活性，但对蔗糖酶-异麦芽糖酶的诱导作用不明显。此外，EGF增强了二肽共转运体的诱导作用。以上结果表明，EGF可促进小肠移植后肠粘膜结构和功能的恢复，促进移植肠的适应性。

Previous reports indicated that epidermal growth factor (EGF) has crucial role for graft adaptation in the rat small bowel transplantation. However, a little is known EGF influences various biological functions in intestinal graft except glucose absorption.The transplanted animals were treated with EGF or saline for one week. Effects of EGF on activities of disaccharidase and the induction of dipeptide cotransporter were investigated in addition to glucose absorption and intestinal histology.Levels of villous height and crypt depth in the EGF-treated group were significantly higher than those in the EGF-untreated group. EGF markedly increased glucose absorption and the induction of sodium glucose cotransporter (SGLT1) in the transplanted animals. EGF also stimulated the activities of disaccharidase such as maltase and sucrase, but it had no effect on the induction of sucrase-isomaltase. Further, EGF enhanced the induction of dipeptide cotransporter. These results demonstrate that EGF accelerates the recovery of mucosal structure and function, and intestinal allograft adaptation after small bowel transplantation.