Triple Quadrupole-Mass Spectrometer

我们正在申请最新一代的三重四极质谱计。它将用于科隆大学“卓越群体：衰老相关疾病中的细胞应激反应”(CECAD)的中央“脂质组学/代谢组学核心设施”，并将安装在CECAD研究中心该设施的一个实验室。在那里，该设施将把光谱仪用于科隆大学其用户群体正在进行和计划的项目，也将用于外部学术研究小组的项目。从长远来看，新的质谱计将取代其已有10年历史的前身型号，该设施已非常成功地将其用于生物样品中脂质的定向定量。该设施最近扩大了服务分析，包括对极性和离子代谢物的有针对性的量化，导致对复杂生物样本中的小代谢物分析的需求大幅增加，如各种模式生物、细胞、组织、体液和分离的细胞细胞器。与其前身相比，所要求的质谱计不仅在正离子模式下具有极高的灵敏度，而且在负离子模式下也具有极高的灵敏度。这对于检测极性和离子代谢物至关重要，与大多数脂质物种不同，这些代谢物通常必须以负离子模式进行测量，并以极低的浓度存在于生物样品中。所需光谱仪的快速极性切换功能允许同时检测脂类和极性代谢物。此外，检测器的大线性动态范围使得只需一次进样即可对低浓度代谢物和非常丰富的化合物进行可靠的定量。这对于生物样品中大量代谢物的高通量定量是至关重要的，因为生物样品中的物质数量通常非常有限。总体而言，所要求的质谱计以最佳方式满足了对复杂生物样品中的小代谢物进行定向量化的仪器的要求，并将使CECAD类脂组学/代谢组学设施能够满足对定量代谢组学分析日益增长的需求。

We are applying for a triple quadrupole-mass spectrometer of the latest generation. It is intended for use in the central "Lipidomics/Metabolomics Core Facility" of the "Cluster of Excellence: Cellular Stress Responses in Aging-Associated Diseases" (CECAD) at the University of Cologne and will be installed in a laboratory of the Facility in the CECAD Research Center. There, the Facility will use the spectrometer for ongoing and planned projects of its user groups at the University of Cologne, but also for projects of external academic research groups. In the long term, the new mass spectrometer will replace its 10-year-old predecessor model, which the Facility has used very successfully for targeted quantifications of lipids in biological samples. The recent expansion of the Facility's service analyses to include targeted quantification of polar and ionic metabolites has led to a strong increase in demand for small metabolite analyses in complex biological samples such as various model organisms, cells, tissues, body fluids, and isolated cell organelles. Compared to its predecessor, the requested mass spectrometer is characterized by its extremely high sensitivity not only in the positive but also in the negative ion mode. This is crucial for the detection of polar and ionic metabolites, which, in contrast to most lipid species, usually have to be measured in the negative ion mode and are present in biological samples in very low concentrations. The fast polarity switching capabilities of the requested spectrometer allows simultaneous detection of lipids and polar metabolites. In addition, the large linear dynamic range of the detector enables robust quantification of both, metabolites at low concentrations and very abundant compounds with a single sample injection. This is critical for high throughput quantifications of large numbers of metabolites in biological samples, where the amount of material is typically very limited. Overall, the requested mass spectrometer optimally fulfils the requirements for an instrument for the targeted quantification of small metabolites in complex biological samples and will enable the CECAD Lipidomics/Metabolomics Facility to meet the increasing demand for quantitative metabolomics analyses.