Study on the role of inhibin-action system in the phisiology and pathophysiology of follicular growth

1.检测体外受精-胚胎移植患者颗粒细胞(GCs)中抑制素、激活素和卵泡抑素基因的表达水平，并分析其与临床参数的关系。因此，在含有成熟卵母细胞的卵泡中，抑制素α亚单位的mR.NA水平较高。然而，在获得高质量胚胎的卵泡中，这一比例较低。这表明抑制素a亚基的表达是卵母细胞成熟所必需的，但过多的表达会对胚胎产生负面影响。2.无论是正常月经周期妇女还是多囊卵巢综合征(PCOS)患者，均经阴道抽吸卵泡，获得卵泡液和卵泡细胞。检测抑制素(A或B)、激活素A、游离卵泡抑素和几种类固醇激素(雄烯二酮、雌二醇、孕酮)的蛋白水平，以及抑制素亚单位(a、3A、(3B)、卵泡抑素288和315亚型)的蛋白水平。正常周期妇女小卵泡卵泡中雄烯二酮含量较高，雌二醇含量较低。随着卵泡的生长，Fonner减小，而Fonner增大。ITT-ibin A和孕酮均随时间延长而升高。卵泡生长，而抑制素B、激活素A、游离卵泡抑素水平无明显变化。在rnRNA水平上，抑制素a和3A亚单位均随卵泡生长而增加，而抑制素(3B亚单位)和卵泡抑素288/315无明显变化。PCOS组GCs中抑制素亚单位-a和(3A)的NINA水平均显著低于大小匹配的卵泡。其余各组间差异均不显著。提示抑制素/激活素系统可能参与了PCOS的病理生理过程。

1.The expression levels of inhibin, activin, and follistatin mRNA were measured in granulose cells (GCs) obtained from IVF-ET patients, which were consequently evaluated if there was any relationship with several clinical parameters. As a result inhibin a-subunit mR.NA level was higher in follicles which contained maturing oocytes. However it was lower in follicles from which high quality embryos were obtained. This suggests though expression of inhibin a=subunit is necessary for oocyte maturation, its too much expression causes negative effect on embryos.2.Follicles of either normal cycling women or polycystic ovarian syndrome (PCOS) patients were transvaginally aspirated and both follicular fluid (FF) and GCs were obtained. Protein levels of inhibin (A or B), activin A, free follistatin and several steroid hormones (androstenedione, estradiol, rogesterone) in FF as well as niR.NA levels of inhibin subunits (a, (3A, (3B), follistatin subtypes (288 and 315) were measured. In normal cycling women androstenedione was high, while estradiol was low in FF of small follicles. As follicles grew, the fonner decreased, while the latter increased. Both itt ibin A and progesterone showed increase following. follicular growth, whereas inhibin B, activin A, free follistatin levels did not change significantly. In the levels of rnRNA, both inhibin a and (3A-subunit showed increase along with follicular growth, whereas neither inhibin (3B-subunit nor follistatin 288/315 changed significantly. In PCOS GCs ni NA levels of both inhibin subunit-a and (3A were significantly lower compared to those of size matched follicles. The other mRNA did not show significant difference. These findings imply the possible involvement of inhibin/activtn system in pathophysiology of PCOS.