Diagnosis of oral cancer by ^<11>C-Choline PET

使用氟-18氟脱氧葡萄糖（FDG PET）的正电子发射断层扫描可用于检测恶性肿瘤复发和评价治疗反应。近年来，~<11>（13）C-胆碱作为另一种肿瘤示踪剂被引入，并成功地显示了恶性肿瘤.本研究旨在探讨~ 1C-胆碱PET临床应用的可能性<11>。HeLa <11>S3细胞摄取~（133）C-胆碱与细胞周期时相的关系，以及它们与常规示踪剂FDG和~（133）<11>C-蛋氨酸（Met）的比较<11>。2-将二甲基氨基乙醇上样到Sep-Pak Accell CM柱上，随后捕获13 <11>C-碘甲烷。结果表明，<11>合成结束时的^ C胆碱平均含量为140 mCi，放化纯度大于99%。该方法简单，使用一次性材料进行纯化，有利于临床常规使用。HeLa S3细胞在S期和G2/M期的FDG摄取明显高于G1期。此外，<11>在G2/M期，Met和β C-胆碱摄取较高。已经得出结论，细胞周期依赖性反映在<11>肿瘤组织的PET成像期间观察到的FDG、Met和13 C-胆碱的摄取中。此外，<11>对兔肿瘤模型给药β-C-Choline，在PET中进行观察，精确地识别肿瘤部位，并在β-C-Choline-PET中显示肿瘤的成像可以应用<11>。

Positron emission tomography using fluorine-18 fluoro-deoxyglucose (FDG PET) is useful of the detection of malignant tumor recurrences and for the evaluation of a therapeutic response. Recently, ^<11>C- Choline was introduced as another tumor tracer and successfully visualized malignant tumor. In this research, we evaluated possibility to clinical application of ^<11>C-Choline PET. The relationship between ^<11>C-choline uptake and the cell cycle phase in HeLa S3 cells, as well as how they compare to the conventional tracer FDG and ^<11>C-Methionine (Met)At first we constructed a computer -controlled apparatus of ^<11>C-Choline synthesis for routine clinical application of PET. 2-dimethylaminoethanol was loaded onto a Sep-Pak Accell CM cartridge, subsequent to the trapping of ^<11>C-methyl iodide. As a result, the average amount of ^<11>C Choline at end of synthesis was 140 mCi, the radiochemical purity was more than 99%. The simplicity of this method and the use of disposable materials for purification way be advantageous for routine clinical use. FDG uptake in HeLa S3 cells was significantly higher in the early S phase and G2/M phase compared to the G1 phase. In addition, Met and ^<11>C-Choline uptakes were higher in the G2/M phase. It has been concluded cell cycle dependency is reflected in the uptake of FDG, Met and ^<11>C-Choline seen during PET imaging of tumor tissue. These results reveal tumor proliferative activity, and can assist in evaluating a therapeutic response.Furthermore, ^<11>C-Choline was administered to a rabbit neoplasm model, and it was observed in PET, and neoplasm locus was identified precisely, and what imaging of neoplasm could apply was shown in ^<11>C-Choline-PET.