Development if a new bone implantation method using recombinant BMP-2 (induced bone implantation)

1. 将重组BMP-2 （rhBMP-2）和冻干同种异体骨移植于骨缺损，比较其修复情况。以胶原蛋白为载体（50 ug），冻干标本，制备直径4 mm的微球。诱导骨植入组将这些颗粒植入腹直肌或大鼠，连续拍摄软x线片。6周后，切除腹直肌内形成的新骨。2 .在同种大鼠颅骨上制备直径为4mm的骨缺损，将诱导骨植入缺损内。取处死大鼠颅骨骨块（直径4mm）冷冻干燥，制备同种异体骨植入组骨移植物。同种异体骨植入组将骨移植物植入大鼠颅骨制备的直径为4mm的骨缺损中。移植后6周，两组大鼠均处死，取移植区，H-E染色，显微镜下观察。在诱导骨植入中，移植物骨和受体床骨之间存在纤维组织和含有毛细血管的结缔组织，并且在受体骨和移植物骨相邻区域部分观察到新的小梁生长。植入6周后，受体床骨与移植物骨间纤维组织毛细血管减少，受体床骨边缘新骨小梁生长更加明显，靠近两根骨的区域出现连接移植物骨与受体床骨的新骨。植入9周后，在受者床骨侧的整个周围观察到新的小梁生长，并且在移植物骨与受者床骨分离的区域也存在孤立的骨组织。没有发现移植物骨吸收的迹象。同种异体骨植入组移植骨与受体床骨之间仅填充纤维结缔组织，植入后3、6、9周未见受体床骨生长。这些结果表明BMP-2诱导骨植入与同种异体骨植入相比是有用的。少

1. We implanted bone induced by recombinant BMP-2 (rhBMP-2) or freeze-dried allogenic bone to bone defect and compared the restoration state.2. Collagen was used as carrier for rhBMP-2 (50 ug), the specimens were freeze-dried, and pellets (4 mm in diameter) were prepared. These pellets were implanted into the rectus abdominis muscle or rats in the induced bone implantation group, and soft X-ray films were serially obtained. After 6 weeks, new bone formed in the rectus abdominis muscle was removed. A bone defect (4 mm in diameter) was prepared in the skull of the same rats, and the induced bone was implanted into the defect.3. Bone pieces (4 mm in diameter) collected from the skull of killed rats were freeze-dried, and bone grafts in the allogenic bone implantation group were prepared. The bone graft was implanted into a bone defect (4 mm in diameter) prepared in the skull of rats in the allogenic bone implantation group.4. Six weeks after grafting, the rats in both groups were killed, … More and the graft area was removed, stained with H-E, and observed by microscopy.5. In the induced bone implantation, fibrous tissue and connective tissue containing capillaries were present between the graft bone and the recipient bed bone, and new trabecular growth from the recipient bed bone was partially observed specificly in areas adjacent to the recipient and graft bones. Six weeks after implantation, capillaries in the fibrous tissue between the recipient bed bone and graft bone decreased, and new trabecular growth from the margin of the recipient bed bone became more marked, and new bone connecting the graft bone with recipient bed bone was observed in areas close to the two bone. Nine weeks after implantation, new trabecular growth was observed in the entire periphery on the recipient bed bone side, and solitary bone tissue was also present in areas where the graft bone is separated from the recipient bed bone. There were no findings suggesting resorption of the graft bone.In the allogenic bone implantation group, the area between the graft bone and recipient bed bone was filled only with fibrous connective tissue, and no bone growth from the recipient bed bone was observed 3, 6, or 9 weeks after implantation.6. These result suggest the usefulness of BMP-2 induced bone implantation compared to allogenic bone implantation. Less