Non-isotopic method for estimating erythrocyte mean age using erythrocyte creatine

红细胞肌酸是反映红细胞年龄的敏感指标，可用于检测轻度和持续性溶血。脾功能亢进引起的血细胞过度破坏是脾功能亢进的重要证据。我们评估了红细胞肌酸作为肝硬变患者红细胞过度破坏的标志的有用性。脾肿大患者的红细胞肌酸显著高于非脾肿大患者和健康对照组，而健康对照组与非脾肿大患者的红细胞肌酸无显著差异。15例红细胞肌酸异常升高的患者中有14例出现脾肿大。网织红细胞计数在正常对照组与有无脾肿大的患者之间无显著差异。红细胞肌酸与脾大小有较好的相关性。因此，红细胞肌酸可用于评估肝硬变…患者的脾功能亢进。机械瓣膜置换术与轻度血管内溶血有关。为了评估心脏瓣膜功能不全引起的血管内溶血，我们分析了32例二尖瓣和/或主动脉瓣置换术患者的红细胞肌酸，并与心脏瓣膜血流动力学进行了比较。红细胞肌酸及其他溶血标志物包括血清乳酸脱氢酶、结合珠蛋白、间接胆红素和网织红细胞计数在有无反流的患者之间差异无统计学意义。瓣膜置换组患者的红细胞肌酸、血清乳酸脱氢酶、结合珠蛋白与正常对照组比较有显著差异，但总的跨瓣峰值血流速度(二尖瓣血流峰值速度+主动脉瓣血流峰值速度)仅与红细胞肌酸呈显著正相关。提示机械瓣膜置换术患者发生血管内溶血的主要原因是跨瓣血流速度，而不是返流，因此红细胞肌酸可作为评价心脏瓣膜功能不全的指标。较少

Erythrocyte creatine is a sensitive marker of erythrocyte age, and can be used to detect slight and continuous hemolysis. Excessive blood cell destruction caused by increased spleen function is important evidence of hypersplenism. We evaluated the usefulness of erythrocyte creatine as a marker of excessive erythrocyte destruction in patients with liver cirrhosis. The patients with splenomegaly showed significantly higher erythrocyte creatine than those without splenomegaly and healthy controls, but there was no significant difference in erythrocyte creatine between healthy controls and those without splenomegaly. Fourteen of the 15 patients with abnormally high erythrocyte creatine had splenomegaly. There were no significant differences in reticulocyte count between healthy controls and the patients with and without splenomegaly. Erythrocyte creatine showed good correlation with spleen size. Thus, erythrocyte creatine can be used for estimating hypersplenism in patients with liver cirr … More hosis.Cardiac vale replacement with mechanical prosthesis is associated with mild intravascular hemolysis. To estimate the intravascular hemolysis due to cardiac valve dysfunction, we analyzed erythrocyte creatine in 32 patients with prosthetic mitral and/or aortic valve, and the data were compared with cardiac valve flow dynamics. No significant differences in erythrocyte creatine and other hemolytic markers including serum lactic dehydrogenase, haptoglobin, indirect bilirubin and reticulocyte counts were observed between the patients in the presence and absence of regurgitations. Although erythrocyte creatine, serum lactic dehydrogenase and haptoglobin in the patients with prosthetic valve showed significant differences as compared to those in healthy controls, total peak transvalvular flow velocities (peak transmitral flow velocity + peak transaortic flow velocity) had significant correlation with only erythrocyte creatine. These results suggest that the intravascular hemolysis is attributed chiefly to transvalvular flow velocity rather than regurgitation in patients with mechanical valve prosthesis, and hence erythrocyte creatine is a possible marker for assessment of cardiac valve dysfunction. Less