Distribution and accumulation of estrogen-like substances in rivers and seas.

在这项研究中，我们测量了河水中雌激素受体结合物质的总量。应用荧光偏振原理测定了雄性鱼血清中卵黄原蛋白的含量。其结果是：1）在木曾三条河流中，雌激素受体结合物质在下游大量检出。由于淀川下游的情况相当，因此可以推测日本河流的情况也类似。2)明确了污染源是城市生活排水的支流。3)这似乎是雌性化的雄性鲤鱼，栖息在河流和雌性化的雄性Oryzias latipes繁殖在水中的河流。然而，这些在统计学上不显著。4)生活在河口入海口的雄性虾虎鱼完全没有雌性化现象。此外，还对一些化学物质进行了调查，并澄清了以下几点。长良川下游水体中检测到高浓度的雌激素受体结合物双酚A。6)从沿海到河流的海产品中二恶英的含量在数量上都是1 ~ 2种稍有认识的程度，与其他地区调查结果相比，其数值较小，由此可见，河流-海域内分泌干扰物的污染是基于人类生活的副产品。虽然污染在下游有所加强，但在海域，它已被稀释（二恶英集中到某些鱼类）。这些是木曾三条河流和伊势湾沿岸的现状和历史情况。我们需要进一步监测。

In this study, we measured the total amounts of estrogen-receptor-binding substances in river water by Beacon【○!R】 which applied the fluorescence polarization principle, and concentrations of serum vitellogenin from male fishes. As the result, 1) In Kiso three rivers, estrogen-receptor-binding substances were able to be detected abounding in the lower reach. Because it is equivalent in the Yodo River downstream, it can be guessed with the similar situation in rivers in Japan. 2) It becomes clear that the pollution source was discharged tributary which city life drainage. 3) It appears to be feminization of male carp that inhabits the river and feminization of male Oryzias latipes bred in the water in the river. However, these were statistically not significant. 4) In male goby that inhabited the river mouth to sea, there was completely no feminization. In addition, a few chemical substances were also investigated, and following points could be clarified. Bisphenol A, one of estrogen-receptor-binding substances, was detected in high concentration in lower reach of Nagara River. 6) Dioxines of the seafood that inhabits the coast to river were the degrees that the 1 or 2 kinds recognizes a little at the quantity, and it was small value in comparison with the investigation of other regions.From these results, the pollution of the river 〜 sea area by endocrine disruptors is based on the by-product of the human life. Though the pollution strengthens as a downstream, in the sea area, it has been diluted (the dioxin concentrates to some kinds of fish). These are present and historical situation of Kiso three rivers and the Ise Bay coast. We need further monitoring.