Functions of neocortical neurons originating in the ganglionic eminence

基本上所有的新皮质神经元都被认为起源于新皮质心室区。然而，该项目的研究者发现，新皮层中的gaba能神经元起源于神经节隆起，并在胚胎阶段迁移到新皮层。在从神经节隆起向新皮层迁移的过程中，我们找不到任何指导切向细胞迁移的支架。相反，我们在Dil标记的切向迁移细胞上发现了neuropilin-1和2。神经匹林-1和-2、Sema3A和3F的配体在纹状体原基表达，似乎分别排斥神经匹林-1和-2阳性细胞到新皮层。此外，通过VZ中Sema3A和皮质板中Sema3F的表达，分别将neuropili-1阳性细胞和neuropilin-2阳性细胞分离到IZ和皮质板中。Neuropilin-1和Dlx2阳性细胞似乎成为皮质板的中间神经元。Neuropilin-2和Dlx2阳性细胞似乎成为IZ的先驱神经元和少突胶质细胞。一些先驱神经元似乎作为连接新皮层的白质神经元留在了白质中。

Basically all the neocortical neurons have been believed to originate in the neocortical ventricular zone. However, the investigator ofthis project found that the GABAergic neurons in the neocortex originate in the ganglionic eminence and migrate to the neocortex at embryonic stages. On the way to migrate from the ganglionic eminence to the neocortex, we could not found any scaffold for the guidance of the tangential cell migration. Instead we found neuropilin-1 and 2 on the Dil labeled tangentially migrating cells. Ligands of neuropilin-1 and -2, Sema3A and 3F were expressed in the primordium of striatum and seemed to repel the neuropilin-1 and -2 positive cells to the neocortex, respectively. In addition, the neuropili-1 positive cells and the neuropilin-2 positive cells were segregated into the IZ and into the cortical plate by Sema3A expression in the VZ and Sema3F expression in the cortical plate, respectively. Neuropilin-1 and Dlx2 positive cells seemed to become the interneurons in the cortical plate. Neuropilin-2 and Dlx2 positive cells seemed to become the pioneer neurons andoligodendrocyte in the IZ. Some of the pioneer neurons seemedto remain in the white matter as white matter neurons which connectneocortices.