Development of a portable blood purification device using novel functional gel

目的：研制一种分散吸附剂的凝胶（Gelrite）血液净化模块。方法：煮沸生理盐水，溶解琼脂粉。将溶液在无添加剂或活性炭（颗粒或颗粒）的塑料圆柱形硬壳中硬化，底部穿孔，分别组装模块G（仅Gelrite），模块GC （Gelrite与各种数量的木炭）。将硬化后的凝胶用塑料吸管沿纵向方向推力21次。制备水或全血测试液用于设备评估。结果：溴酚蓝（BPB）浓度随着模块G的增加仅下降10%，而随着模块GC中活性炭分散量的增加，BPB浓度逐渐下降，颗粒活性炭分散时间为100小时，颗粒活性炭分散时间为400小时。然后可以通过改变凝胶粉的用量和吸附剂的用量以及吸附剂的大小来控制溶质的去除速率。模块GC连续从牛全血中去除肌酐24小时。结论：将这些模块与小型血液过滤器相结合，可构建便携式人工肾脏系统，以去除多余的水分。

Aim : A blood purification module was developed with gel (Gelrite) in which adsorbent was dispersed.Methods : Normal saline was boiled, and agar powder was dissolved in it. The solution was hardened with no additive or activated charcoal (granular or particulate) in a plastic cylindrical hard shell with a perforated bottom plate to assemble module G (Gelrite only), module GC (Gelrite with various amounts of charcoal), respectively. The hardened gel was thrust with a plastic straw 21 times in the longitudinal flow direction. Aqueous or whole blood test solution was prepared for device evaluation.Results : Bromophenol blue (BPB) concentration decreased only by 10 % with module G, whereas it gradually decreased according to the amount of activated charcoal dispersed in the gel with module GC up to 100 hrs with granular charcoal and 400 hrs with particulate on. Then rates of solute removal may be controlled by changing amount of gel powder and amount of adsorbent as well as the size of the adsorbent. Module GC removed creatinine continuously from bovine whole blood for 24 hours.Conclusions : A portable artificial kidney system may be constructed by combining these modules with a small hemofilter for removing excess water.