Ion mobility mass spectrometer with electrospray ionisation (ESI-IMS-MS) for protein analysis

科隆大学新成立的植物代谢生物化学实验室使用质谱法和生物化学方法的结合来研究环境相互作用背景下的植物代谢过程，重点是蛋白质和蛋白质组学分析。植物代谢生物化学系是植物科学卓越集群（CEPLAS）的一部分，并将与集群的其他小组合作，为各种项目提供蛋白质组学方法。新的研究项目和在新的地点继续运行项目需要拟议的离子迁移率电喷雾质谱仪（ESI-IMS-MS）。植物与生物和非生物环境相互作用中的信号过程是研究的热点。为了可靠地检测和量化蛋白质丰度的动态变化，蛋白质与其他蛋白质或配体的相互作用，以及单个蛋白质的翻译后修饰，需要高灵敏度、高分辨率的质谱仪。除了疏水性（HPLC）分离、质荷比（m/z）分离（四极杆）和质量分析（离子阱/飞行管）分离外，IMS质谱系统还有一个额外的模块，用于分析离子迁移率，并将其作为进一步的分离标准。这第四个分离维度显著增加了分析的多肽数量，从而实现了鉴定蛋白质数量和定量准确性的显著增加。此外，还需要IMS模块能够根据分子的三维结构分离相同质量的分子。首席申请人的工作小组以前在莱布尼茨Universität汉诺威的植物蛋白质组学部门工作。该系有三台用于蛋白质组分析的质谱仪，使小组成员能够获得实践经验，并在植物蛋白质组学方面发展广泛的专业知识。

The newly established plant metabolic biochemistry lab at the University of Cologne uses a combination of mass spectrometry and biochemical approaches to study plant metabolic processes in the context of environmental interactions with a focus on protein and proteome analyses. The Department of Plant Metabolic Biochemistry is part of the Cluster of Excellence on Plant Sciences (CEPLAS) and will contribute proteomic approaches to various projects in cooperation with other groups of the cluster. The proposed ion mobility mass spectrometer with electrospray ionization (ESI-IMS-MS) is required for new research projects and to continue running projects at the new location. Signaling processes in the interaction of plants with their biotic and abiotic environment are the focus of research interest. In order to reliably detect and quantify the dynamics of protein abundances, the interaction of proteins with other proteins or ligands, as well as post-translational modifications at individual proteins, a highly sensitive mass spectrometer with a high resolution is required. In addition to separation by hydrophobicity (HPLC), separation by mass-to-charge (m/z) ratio (quadrupole) and mass analysis (ion trap/flight tube), IMS mass spectrometry systems have an additional module that analyses ion mobility and uses it as a further separation criterion. This fourth dimension of separation significantly increases the number of peptides analyzed and thus achieves a dramatic increase in both the number of proteins identified and the accuracy of quantification. The IMS module is furthermore required to enable the separation of molecules of identical mass based on their three-dimensional structure. The lead applicant's working group was previously based in the Department of Plant Proteomics at Leibniz Universität Hannover. Three mass spectrometers for proteome analysis were available in the department allowing the group members to gain hands-on experience and develop a broad expertise for plant proteomics.