General study of development of periodontal disease examination

我们开发了一种检测牙周病原体的新方法。聚合酶链式反应方法对牙周治疗方案的制定和预后的判断具有重要意义。DNA微阵列也有助于了解牙周疾病的状况。另一方面，我们开发了有用的疾病敏感性诊断方法。Fcγ受体基因调控炎性细胞因子的产生。Fcγ受体基因多态性影响宿主对细菌攻击的反应。组织、唾液或牙周沟液(GCF)中炎症相关因子的分布分析可用于开发新的治疗方法。牙周液中糖胺多糖水平与牙槽骨吸收相关。牙周积液中钙保护素的测定与牙周炎的活动性有关。此外，我们还发现呼气的挥发性硫化物与牙周病密切相关。我们开发了一种利用牙齿固定来检测创伤性咬合的新方法。为了检查牙周炎对身体的影响，我们发现高敏感性的CRP是非常有用的。

We developed a new method to detect periodontal pathogens. PCR method is very useful for making periodontal treatment plan and decision of prognosis. DNA microarray is also useful to know state of periodontal disease. On the other hand, we developed useful diagnosis method for disease sensitivity. Fc γ receptor gene regulates the production of inflammatory cytokines. Fc γ receptor polymorphisms affect the host response to bacterial attack. Profile analysis of inflammatory related factors from tissues, saliva, or gingival crevicular fluids (GCF) is available for the development of new therapy. Glycosaminoglycan level in GCF correlated with alveolar bone resorption. The measurement of calprotectin from GCF is associated with the activity of periodonttitis. Moreover we identified that volatile sulfer compound of expiration is closely correlated with periodontal disease. We developed a new method to detect traumatic occlusion using tooth fixation. To examine the effect of periodontitis for the body, we identified that high sensitivity CRP is very useful.