Removal of Phenolic compounds with an endocrine-disrupting effect by Polymerization and Precipitation Method Using Microbial Peroxidase

1.利用转盘接触器对鸡腿菇产生木质素降解酶过氧化物酶的粗酶活性进行了研究，并考察了诱导剂对过氧化物酶产生的影响。作为诱导剂，丁香醛达嗪没有诱导效果，而过氧化氢或5-氨基乙酰丙酸作为诱导剂时，效果较好。如在培养第3天加入诱导剂，酶活可提高1.8-2倍(100U/ml)。过氧化氢的最佳用量为0.1~0.2 mM。今后，我们必须考虑诱导剂在重复分批培养中的效果。研究了微生物过氧化物酶聚合沉淀法去除酚类化合物(包括内分泌干扰物)的反应条件。考察了过氧化物酶、酚类化合物和过氧化氢的最佳化学计量关系，以及pH和温度对去除效果的影响。在适宜的酶剂量下，过氧化氢与苯酚的最佳比例接近1/1(摩尔/摩尔)，需要12-14U的酶才能去除1 mg的白藜芦醇。在过氧化物酶投加量不足的情况下，最佳pH值为9.0，降低反应温度有利于提高去除效率。此外，加入非离子表面活性剂Triton X-100，去除苯酚所需的过氧化物酶用量减少了约1/4。双酚A去除的反应条件与苯酚相似，但所需的过氧化物酶用量和反应时间是苯酚的4-5倍。

1. The peroxidase (a lignin degrading enzyme) production by Coprinus cinereus using a rotating disk contactor was examined for the purpose of the producing the crude enzyme, and the effect of an inducer on peroxidase production was also investigated. As an inducer, no results were obtained using syringaldazine, but when hydrogen peroxide or 5-amino-levulinic acid were used as the additive to the culture medium, the suitable effectiveness was obtained. For example, adding the inducer into the 3 ^<rd> day culture, the enzyme activity was increased 1.8-2 times (100U/ml). Further, the optimum dosage of hydrogen peroxide was 0.1-0.2 mM. In future, we must consider the effectiveness of the inducer in a repeated batch culture.2. The reaction conditions on phenolic compounds (including an endocrine-disruptor) removal by the polymerization and precipitation method using the microbial peroxidase were investigated. Consequently, the optimum stoichiometric relationship between peroxidase, phenolic compounds and hydrogen peroxide, and the effect of pH and temperature on removal efficiency were examined. The most effective ratio of hydrogen peroxide to phenol was nearly 1/1 (mol/mol) at an adequate enzyme dose, and 12-14 U of the enzyme was needed to remove 1 mg of pehnol. At an insufficient peroxidase dose, the optimum pH value was 9.0, and lowering the reaction temperature led to the improvement of removal efficiency. Moreover, adding non-ionic surfactant (Triton X-100), the required amount of peroxidase to remove phenol was reduced about 1/4. The reaction conditions on bisphenol A removal presented the similar behavior compared to the cases of phenol, but the required amount of peroxidase and reaction time were 4-5 times.