Construction of Catalytic-and Electron Transfer-Systems Using Antibody Matrices

获得了抗电子供体-受体结合物的单抗。将抗电子供体(卟啉)抗体和抗电子受体(醌)抗体的杂交瘤融合，制备杂合抗体。该杂合抗体能同时与卟啉和苯醌结合，并加速了在抗原结合部位从卟啉分子到醌分子的电子传递。抗卟啉抗体与Fe(III)-卟啉形成的络合物催化邻苯三酚氧化。制备了4，4‘-联吡啶-1-(羧基戊基)-1’-甲基-二氯联吡啶的单抗。用基于表面等离子体共振(SPR)的生物传感器技术研究了其中一种抗体与紫精二聚体的络合作用。通过将抗体溶液添加到紫精二聚体-抗体复合体中，可以观察到SPR反应信号强度的增强，这表明抗体和紫精二聚体之间形成了线性超分子。甲基紫精通过抑制抗体与紫精二聚体之间的络合物形成，以及抗体与紫精二聚体形成超分子的信号增强来实现对甲基紫精传感过程的定量放大。该体系的灵敏度比在固定在传感器芯片表面的抗体中加入甲基紫精的灵敏度高两个数量级。利用抗体设计合成了新型树枝状大分子。使用了免疫球蛋白M(IgM)，它是通过附加肽连接的免疫球蛋白G的五聚体、免疫球蛋白G和化学修饰的免疫球蛋白G。将卟啉的IgM与修饰后的IgG混合，形成树枝状超分子。抗体通过非共价键与抗体的特异性分子识别相联系。

Monoclonal antibodies for an electron donor-acceptor conjugate were obtained. The hybrid antibody was also prepared by fusing the hybridomas of anti-electron donor (porphyrin) antibody and anti-electron acceptor (quinone) antibody. The hybrid antibody was found to bind both porphyrin and quinone simultaneously, and accelerated the electron transfer from porphyrin to quinone molecule in the antigen-combining site. The complex of the anti-porphyrin antibody with Fe(III)-porphyrin catalyzed oxidation of pyrogallol. The peroxidase activity of this system was found to be high as that of HRP.Monoclonal antibodies for 4,4'-bipyridiniums 1-(carboxypentyl)-1'-methyl-dichloride have been prepared. The complex formation of one of the antibodies with viologen dimer has been studied by a biosensor technique based on surface plasmon resonance (SPR). Enhancement of response signal intensities in SPR is observed by the addition of the antibody solution to the viologen dimer-antibody complex indicating the formation of linear supramolecules between the antibody and viologen dimer. The quantitative amplification of methyl viologen sensing processes is realized by the inhibition of the complex formation between antibodies and viologen dimer by methyl viologen and signal enhancement due to the supramolecular formation of the antibody with viologen dimer. The sensitivity in this system is found to be two orders larger than that in the simple addition of methyl viologen to the antibody immobilized to the surface of the sensor chip.Novel dendrimers have been designed and synthesized by using antibodies. Immunoglobulin M (IgM) which is a pentamer of IgG linked by additional peptides, IgG, and chemically modified IgG were used. Mixing IgM for porphyrin and IgG modified with porphyrin molecule leads to formation of dendritic supramolecules. Antibodies were linked by noncovalent bond with specific molecular recognition of antibodies.