Basic study on the development of "Bio-Defence Foods" by DNA motifs from dairy lactic acid bacteria

发酵牛奶及其相关乳制品乳酸菌一直被认为对健康有价值。近年来，对乳制品乳酸菌(LAB)，特别是益生菌及其衍生物的生理功能进行了验证性实验。免疫调节等生物学功能已引起人们的极大兴趣。在我们最近的研究中，我们特别关注实验室中的DNA作为实验室免疫调节因子之一。本研究旨在阐明肠道DNA对细胞因子的诱导作用。用实验室提取的DNA基序刺激猪Peyer‘s结和肠系膜淋巴结后，用实时荧光定量聚合酶链式反应(Real-time PCR)方法检测细胞因子mRNA的诱导。此外，还研究了L糖浆的一种新功能。DNA基序可增强Peyer‘s斑块中TH1型细胞因子(IL-6、IL-12、IFNy)的表达。L乳酸菌JCM1131T培养上清液诱导巨噬细胞趋化作用是由益生菌乳酸菌所发挥的一种新的生物学功能，其趋化因子命名为“Gserokine”。因此，该活性有望作为益生菌免疫调节特性的功能参数之一。本研究证明了该实验室的免疫刺激DNA基序在肠道中发挥了细胞因子的诱导作用，并提出该实验室是生产新型功能食品--生物防御食品的发酵剂的良好候选者。

Fermented milks and their related dairy lactic acid bacterla have long been thought to be of value for health maintenance. Recently, corroborative experiments on the physiological functions of dairy lactic acid bacteria (LAB), especially the probiotic LAB, and their derivatives have been carried out. A great interest has been focused on biological functions such as immunomodulations. In our recent study, we have been particularly focusing on the DNA from LAB as one of the immunomoduratory factors in the LAB. The present study was conducted to elucidate the cytokine induction by the DNA in the gut. After stimulation of swine peyer's patch and mesenteric lymph node wlth DNA motifs from the LAB, induction of cytokine mRNA was estimated by real time PCR method. In addition, a novel function of L gasseri was investigated. The DNA motifs augmented induction of TH1 type cytokine (IL-6, IL-12, IFNy) mRNA in peyer's patches. The macrophage clemotaxis induced by L gasseri JCM1131^T culture supematants, which the chemotactic factor was designated as "Gasserokine", was discovered to be a new biological function exerted by probiotic lactic acid bacteria. Therefore, the activity is expected to be used for one of the functional parameters in the immunomodulating properties of probiotic lactic acid bacteria. This study demonstrated that immunostimulatory DNA motif from the LAB exerted the cytokine induction in the gut and proposed that the LAB is a good candidate of a starter culture for the production of new functional foods, "Bio-Defense Foods".