Characterization of host and bacterial factors affecting the exit of uropathogenic Escherichia coli from intracellular bacterial communities in bladder epithelial cells

我们的目的是通过加深对UPEC-尿路上皮细胞相互作用的理解来揭示复发性UTI的机制关系。整合我们对UPEC细胞内生命周期和尿路上皮细胞退出的知识，可能会改善复发性UTI患者的诊断和新的治疗策略。我们的目的是了解决策过程和机制控制出口的UPEC从他们的细胞内生态位在膀胱上皮细胞。尽管已知某些细菌因子参与UPEC细胞内存活和IBC形成，但关于内体途径的哪些宿主细胞调节剂参与的机制细节以及晚期内体/溶酶体隔室在细胞内生长的UPEC的吞噬体逃逸的背景下的作用仅不完全了解。

Our aim is to uncover mechanistic relationships underlying recurrent UTI by deepening the understanding of the UPEC-urothelial cell interplay. Integration of our knowledge on the UPEC intracellular life cycle and urothelial cell exit might lead to improved diagnostic and novel treatment strategies for patients suffering from recurrent UTI. We aim to understand decision making processes and mechanisms controlling the exit of UPEC from their intracellular niche in bladder epithelial cells. Although it is known that certain bacterial factors are involved in UPEC intracellular survival and IBC formation, the mechanistic details regarding which host cell modulators of the endosomal pathway are involved, and the role of the late endosomal/lysosomal compartment in the context of the phagosomal escape of intracellularly growing UPEC are only incompletely understood.