Identification of virulent factors related to rearrangement of skeleton Proteins in mycobacterial infections.

目的鉴定结核分枝杆菌、马红球菌等胞内细菌的新毒力因子。1细胞感染强毒或无毒R.用免疫细胞学方法进行观察。光学显微镜和透射电镜观察表明，毒力菌增加为单膜空泡。共聚焦荧光显微镜观察与内体标记物和溶酶体标记物显示，大多数吞噬体含有毒力细菌与溶酶体融合。监测含R. equi，感染R.马用亲酸探针染色。含无毒R. Equi变成了酸性的。与此相反，毒力R.对毒力R. equi抑制了巨噬细胞中酸性隔室的酸化。这些结果表明，强毒R.马能通过分泌抑制吞噬溶酶体酸化的物质在巨噬细胞的吞噬溶酶体环境中生存。

To identify new virulent factors of intracellular bacteria, such as Mycobacterium tuberculosis and Rhodococcus equi, murine macrophage-like J774. 1 cells were infected with virulent or avirulent R. equi and observed by using immunocytological methods. Observations by light microscopy and transmission electron microscopy showed that the virulent bacteria increased into single membrane vacuoles. Observations by confocal fluorescence microscopy with an endosomal marker and lysosomal markers revealed that most phagosomes containing virulent bacteria were fused with lysosomes. To monitor acidification of phagosomes containing R. equi, the cells infected with R. equi were stained with acidotropic probe. The phagosomes containing avirulent R. equi became acidic. In contrast, virulent R. eqiu did not change pH. The medium of virulent R. equi was suppressed acidification of acidic compartments in macrophages. These results suggested that virulent R. equi can survive phagolysosomal environment in macrophages by secreting substance which suppress acidification of phagolysosome.